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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 7 — Jul. 17, 2006

Determination of scattering volume fraction and particle size distribution in the superficial layer of a turbid medium by using diffuse reflectance spectroscopy

Yasser S. Fawzy and Haishan Zeng  »View Author Affiliations

Applied Optics, Vol. 45, Issue 16, pp. 3902-3912 (2006)

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We have investigated the possibility of determining changes in the volume fraction of microstructure scatterers in the superficial tissue layers by using diffuse reflectance spectroscopy. To that extent we have built a two-layer optical phantom by using microparticles with various sizes in order to simulate the scattering properties of tissue microstructures. Reflectance spectral measurements were performed on a number of optical phantoms having different volume fractions of various microparticle sizes. An analytical model was developed using light-transport theory and fractal modeling approaches and was then fitted to the measured reflectance to calculate the volume fractions of the microparticles in phantoms. The results showed that we could measure changes in both the total volume fraction of the microparticles and in the overall size distribution of the microparticles with good accuracy ( > 80 % ) . These results suggest the potential of using this method for measuring the volume fraction changes of tissue microstructure scatterers and applications in the detection of cancerous related morphological and structural changes.

© 2006 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(290.0290) Scattering : Scattering
(290.5850) Scattering : Scattering, particles
(290.7050) Scattering : Turbid media

Original Manuscript: April 14, 2005
Revised Manuscript: August 16, 2005
Manuscript Accepted: November 3, 2005

Virtual Issues
Vol. 1, Iss. 7 Virtual Journal for Biomedical Optics

Yasser S. Fawzy and Haishan Zeng, "Determination of scattering volume fraction and particle size distribution in the superficial layer of a turbid medium by using diffuse reflectance spectroscopy," Appl. Opt. 45, 3902-3912 (2006)

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