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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 9 — Sep. 26, 2007

Sample holder and methodology for measuring the reflectance and transmittance of narrow-leaf samples

Scott D. Noble and Trever G. Crowe  »View Author Affiliations

Applied Optics, Vol. 46, Issue 22, pp. 4968-4976 (2007)

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Measuring the reflectance and transmittance of narrow samples can be difficult, as the width of the illuminating beam may be greater than the width of the sample. The small sample area can also compound the already time-consuming process of reconfiguring the instrument between reflectance and transmittance measurements by introducing additional alignment problems. A method of measuring the reflectance and transmittance properties of narrow-leaf samples using reflectance configurations only is developed and tested. The method uses a mask and mask correction and relationships between reflectance measurements against contrasting backgrounds to determine sample reflectance and transmittance. The design of the accompanying sample-holding apparatus is also described. In testing, the mean error was less than 1% reflectance∕transmittance, and standard deviation of the error was approximately 1% reflectance and 2% transmittance as compared to samples measured using conventional measurement configurations.

© 2007 Optical Society of America

OCIS Codes
(000.2170) General : Equipment and techniques
(120.3930) Instrumentation, measurement, and metrology : Metrological instrumentation
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(280.0280) Remote sensing and sensors : Remote sensing and sensors
(300.0300) Spectroscopy : Spectroscopy

ToC Category:
Instrumentation, Measurement, and Metrology

Original Manuscript: October 16, 2006
Revised Manuscript: May 8, 2007
Manuscript Accepted: May 16, 2007
Published: July 6, 2007

Virtual Issues
Vol. 2, Iss. 9 Virtual Journal for Biomedical Optics

Scott D. Noble and Trever G. Crowe, "Sample holder and methodology for measuring the reflectance and transmittance of narrow-leaf samples," Appl. Opt. 46, 4968-4976 (2007)

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  1. S. D. Noble, "Crop and weed leaf reflectance and classification," M.S. thesis (University of Saskatchewan, Saskatoon, SK, 2002).
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