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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 9 — Sep. 26, 2007

Adaptive interferometry of protein on a BioCD

Leilei Peng, Manoj M. Varma, Wonryeon Cho, Fred E. Regnier, and David D. Nolte  »View Author Affiliations


Applied Optics, Vol. 46, Issue 22, pp. 5384-5395 (2007)
http://dx.doi.org/10.1364/AO.46.005384


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Abstract

Adaptive spinning-disk interferometry is capable of measuring surface profiles of a thin biolayer with subnanometer longitudinal resolution. High-speed phase modulation in the signal beam arises from the moving surface height profile on the spinning disk and is detected as a homodyne signal via dynamic two-wave mixing. A photorefractive quantum-well device performs as an adaptive mixer that compensates disk wobble and vibration while it phase-locks the signal and reference waves in the phase quadrature condition ( π / 2 relative phase between the signal and local oscillator). We performed biosensing of immobilized monolayers of antibodies on the disk in both transmission and reflection detection modes. Single- and dual-analyte adaptive spinning-disk immunoassays were demonstrated with good specificity and without observable cross-reactivity. Reflection-mode detection enhances the biosensing sensitivity to one-twentieth of a protein monolayer, creates a topographic map of the protein layer, and can differentiate monolayers of different species by their effective optical thicknesses.

© 2007 Optical Society of America

OCIS Codes
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(160.5320) Materials : Photorefractive materials
(170.3890) Medical optics and biotechnology : Medical optics instrumentation

ToC Category:
Interferometry

History
Original Manuscript: February 26, 2007
Manuscript Accepted: April 18, 2007
Published: July 23, 2007

Virtual Issues
Vol. 2, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Leilei Peng, Manoj M. Varma, Wonryeon Cho, Fred E. Regnier, and David D. Nolte, "Adaptive interferometry of protein on a BioCD," Appl. Opt. 46, 5384-5395 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-46-22-5384

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