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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 3 — Mar. 7, 2007

Improving resolution in microscopic holography by computationally fusing multiple, obliquely illuminated object waves in the Fourier domain

Jeffery R. Price, Philip R. Bingham, and C. E. Thomas, Jr.  »View Author Affiliations


Applied Optics, Vol. 46, Issue 6, pp. 827-833 (2007)
http://dx.doi.org/10.1364/AO.46.000827


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Abstract

We present a computational method to increase the effective NA of a holographic microscopy system operating in air. Our optical system employs a reflection Mach–Zender architecture and computational reconstruction of the full complex (phase and amplitude) wavefront. Based on fundamental diffraction principles, different angles of incident illumination result in different diffracted orders of the object wave being imaged. We record, store, and computationally recombine these object waves to expand the spatial frequency response. Experimental results demonstrate an improvement in the effective NA of our system from 0.59 to 0.78.

© 2007 Optical Society of America

OCIS Codes
(090.1760) Holography : Computer holography
(090.2880) Holography : Holographic interferometry

ToC Category:
Holography

History
Original Manuscript: August 21, 2006
Revised Manuscript: October 6, 2006
Manuscript Accepted: October 26, 2006
Published: February 2, 2007

Virtual Issues
Vol. 2, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Jeffery R. Price, Philip R. Bingham, and C. E. Thomas, Jr., "Improving resolution in microscopic holography by computationally fusing multiple, obliquely illuminated object waves in the Fourier domain," Appl. Opt. 46, 827-833 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-46-6-827

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