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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 4 — Apr. 23, 2008

Hyperspectral Fourier transform spectrometer for reflection spectroscopy and spectral self-interference fluorescence microscopy

Julia Rentz Dupuis, James Needham, Emre Özkumur, David A. Bergstein, Bennett B. Goldberg, James R. Engel, David L. Carlson, and M. Selim Ünlü  »View Author Affiliations


Applied Optics, Vol. 47, Issue 9, pp. 1223-1234 (2008)
http://dx.doi.org/10.1364/AO.47.001223


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Abstract

A hyperspectral Fourier transform spectrometer has been developed for studying biological material bound to optically reflecting surfaces. This instrument has two modes of operation: a white-light reflection mode and a spectral self-interference fluorescence mode. With the combined capability, information about the conformation of an ensemble of biomolecules may be determined. To the best of our knowledge, ours is the first report of this hybrid white-light reflection, spectral self-interference fluorescence measurement with any type of hyperspectral imager. The measurement technique is presented along with a full description of the system, including theoretical performance projections. Proof-of-principle measurements of artificial samples are shown, and the results are discussed.

© 2008 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: September 27, 2007
Revised Manuscript: December 12, 2007
Manuscript Accepted: January 9, 2008
Published: March 17, 2008

Virtual Issues
Vol. 3, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Julia Rentz Dupuis, James Needham, Emre Özkumur, David A. Bergstein, Bennett B. Goldberg, James R. Engel, David L. Carlson, and M. Selim Ünlü, "Hyperspectral Fourier transform spectrometer for reflection spectroscopy and spectral self-interference fluorescence microscopy," Appl. Opt. 47, 1223-1234 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-47-9-1223

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