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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 9 — Sep. 4, 2009

Microscopic hyperspectral imaging used as a bio-optical taxonomic tool for micro- and macroalgae

Zsolt Volent, Geir Johnsen, and Fred Sigernes  »View Author Affiliations


Applied Optics, Vol. 48, Issue 21, pp. 4170-4176 (2009)
http://dx.doi.org/10.1364/AO.48.004170


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Abstract

In the presented study a hyperspectral imager ( 400 700 nm ) mounted on a stereo-microscope was used to separate differences in in vivo optical signatures identifying different pigment groups of bloom-forming phytoplankton and macroalgae by comparing spectral absorption, transmittance, and reflectance from 400 700 nm . The results show that the hyperspectral imager could be used to detect spectral characteristics on the μm level to calibrate, validate, identify, and separate objects with differences in color (optical fingerprinting). This information can be used for pigment group specific taxonomy (bio-optical taxonomy), eco-physiological information (e.g., health status), monitoring, and mapping applications.

© 2009 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.4234) Imaging systems : Multispectral and hyperspectral imaging

ToC Category:
Imaging Systems

History
Original Manuscript: January 22, 2009
Revised Manuscript: June 26, 2009
Manuscript Accepted: June 26, 2009
Published: July 14, 2009

Virtual Issues
Vol. 4, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Zsolt Volent, Geir Johnsen, and Fred Sigernes, "Microscopic hyperspectral imaging used as a bio-optical taxonomic tool for micro- and macroalgae," Appl. Opt. 48, 4170-4176 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-48-21-4170


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