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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 10 — Jul. 19, 2010

Two-photon fluorescence of Coumarin 30 excited by optimally shaped pulses

Milan P. Poudel, Alexandre A. Kolomenskii, and Hans A. Schuessler  »View Author Affiliations


Applied Optics, Vol. 49, Issue 16, pp. 3075-3081 (2010)
http://dx.doi.org/10.1364/AO.49.003075


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Abstract

We optimized the two-photon fluorescence (TPF) of a Coumarin 30 dye by using a feedback-controlled femtosecond pulse shaping technique. For optimization we implemented an evolutionary algorithm with a liquid crystal phase-only pulse shaper in a folded 4f setup. The optimization procedure applied to the second harmonic generation, and TPF noticeably improved the output signals and demonstrated good convergence. In addition, signal ratios involving TPF and second harmonic generation (SHG) were successfully optimized. The correlation between TPF and SHG was studied, and it was found to decrease when the pulse shape was close to the optimum. These experimental results are of interest for potential applications of coherent control to complex molecular systems as well as in biomedical imaging.

© 2010 Optical Society of America

OCIS Codes
(190.7110) Nonlinear optics : Ultrafast nonlinear optics
(320.5520) Ultrafast optics : Pulse compression
(320.5540) Ultrafast optics : Pulse shaping
(350.5030) Other areas of optics : Phase

ToC Category:
Nonlinear Optics

History
Original Manuscript: December 23, 2009
Revised Manuscript: April 22, 2010
Manuscript Accepted: May 1, 2010
Published: May 26, 2010

Virtual Issues
Vol. 5, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Milan P. Poudel, Alexandre A. Kolomenskii, and Hans A. Schuessler, "Two-photon fluorescence of Coumarin 30 excited by optimally shaped pulses," Appl. Opt. 49, 3075-3081 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-49-16-3075

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