OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 10 — Jul. 19, 2010

Measurement of two-photon excitation spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy

Hiroshi Hashimoto, Keisuke Isobe, Akira Suda, Fumihiko Kannari, Hiroyuki Kawano, Hideaki Mizuno, Atsushi Miyawaki, and Katsumi Midorikawa  »View Author Affiliations


Applied Optics, Vol. 49, Issue 17, pp. 3323-3329 (2010)
http://dx.doi.org/10.1364/AO.49.003323


View Full Text Article

Enhanced HTML    Acrobat PDF (716 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We present measurements of two-photon excitation (TPE) spectra of various fluorescent proteins with nonlinear Fourier-transform spectroscopy. By using an ultrabroadband laser pulse with a spectrum ranging from 700 to 1100 nm , the absolute TPE spectra of six typical fluorescent proteins (SeBFP, Sapphire, eGFP, eCFP, Venus, DsRed) were measured with high spectral resolution.

© 2010 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(300.6420) Spectroscopy : Spectroscopy, nonlinear

ToC Category:
Spectroscopy

History
Original Manuscript: January 27, 2010
Revised Manuscript: May 7, 2010
Manuscript Accepted: May 14, 2010
Published: June 7, 2010

Virtual Issues
Vol. 5, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Hiroshi Hashimoto, Keisuke Isobe, Akira Suda, Fumihiko Kannari, Hiroyuki Kawano, Hideaki Mizuno, Atsushi Miyawaki, and Katsumi Midorikawa, "Measurement of two-photon excitation spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy," Appl. Opt. 49, 3323-3329 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-49-17-3323


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248, 73–76 (1990). [CrossRef] [PubMed]
  2. C. Xu, W. Zipfel, J. B. Shear, R. M. Williams, and W. W. Webb, “Multiphoton fluorescence excitation: New spectral windows for biological nonlinear microscopy,” Proc. Natl. Acad. Sci. USA 93, 10763–10768 (1996). [CrossRef] [PubMed]
  3. K. Isobe, W. Watanabe, S. Matsunaga, T. Higashi, K. Fukui, and K. Itoh, “Multi-spectral two-photon excited fluorescence microscopy using supercontinuum light source,” Jpn. J. Appl. Phys. 44, L167–L169 (2005). [CrossRef]
  4. P. Allcock and D. L. Andrews, “Two-photon fluorescence: resonance energy transfer,” J. Chem. Phys. 108, 3089–3095 (1998). [CrossRef]
  5. G. Y. Fan, H. Fujisaki, A. Miyawaki, R. K. Tsay, R. Y. Tsien, and M. H. Ellisman, “Video-rate scanning two-photon excitation fluorescence microscopy and ratio imaging with Cameleons,” Biophys. J. 76, 2412–2420 (1999). [CrossRef] [PubMed]
  6. G. A. Blab, P. H. M. Lommerse, L. Cognet, G. S. Harms, and T. Schmidt, “Two-photon excitation action cross-sections of the autofluorescent proteins,” Chem. Phys. Lett. 350, 71–77 (2001). [CrossRef]
  7. W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21, 1369–1377 (2003). [CrossRef] [PubMed]
  8. E. Spiess, F. Bestvater, A. Heckel-Pompey, K. Toth, M. Hacker, G. Stobrawa, T. Feurer, C. Wotzlaw, U. Berchner-Pfannschmidt, T. Porwol, and H. Acker, “Two-photon excitation and emission spectra of the green fluorescent protein variants ECFP, EGFP and EYFP,” J. Microsc. 217, 200–204 (2005). [CrossRef] [PubMed]
  9. M. Drobizhev, S. Tillo, N. S. Makarov, T. E. Hughes, and A. Rebane, “Absolute two-photon absorption spectra and two-photon brightness of orange and red fluorescent proteins,” J. Phys. Chem. B 113, 855–859 (2009). [CrossRef] [PubMed]
  10. H. Hosoi, S. Yamaguchi, H. Mizuno, A. Miyawaki, and T. Tahara, “Hidden electronic excited state of enhanced green fluorescent protein,” J. Phys. Chem. B 112, 2761–2763 (2008). [CrossRef] [PubMed]
  11. M. Bellini, A. Bartoli, and T. W. Hänsch, “Two-photon Fourier spectroscopy with femtosecond light pulses,” Opt. Lett. 22, 540–542 (1997). [CrossRef] [PubMed]
  12. J. P. Ogilvie, K. J. Kubarych, A. Alexandrou, and M. Joffre, “Fourier transform measurement of two-photon excitation spectra: applications to microscopy and optimal control,” Opt. Lett. 30, 911–913 (2005). [CrossRef] [PubMed]
  13. K. Isobe, A. Suda, M. Tanaka, F. Kannari, H. Kawano, H. Mizuno, A. Miyawaki, and K. Midorikawa, “Multifarious control of two-photon excitation of multiple fluorophores achieved by phase modulation of ultra-broadband laser pulses,” Opt. Express 17, 13737–13746 (2009). [CrossRef] [PubMed]
  14. K. Isobe, A. Suda, M. Tanaka, F. Kannari, H. Kawano, H. Mizuno, A. Miyawaki, and K. Midorikawa, “Fourier-transform spectroscopy combined with a 5fs broadband pulse for multispectral nonlinear microscopy,” Phys. Rev. A 77, 063832(2008). [CrossRef]
  15. T. Nagai, K. Ibata, E. Sun Park, M. Kubota, K. Mikoshiba, and A. Miyawaki, “A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications,” Nat. Biotechnol. 20, 87–90 (2002). [CrossRef]
  16. J. K. Ranka, A. L. Gaeta, A. Baltuska, M. S. Pshenichnikov, and D. A. Wiersma, “Autocorrelation measurement of 6fs pulses based on the two-photon-induced photocurrent in a GaAsP photodiode,” Opt. Lett. 22, 1344–1366 (1997). [CrossRef]
  17. A. Baltuska, M. S. Pshenichnikov, and D. A. Wiersma, “Amplitude and phase characterization of 4.5fs pulses by frequency-resolved optical gating,” Opt. Lett. 23, 1474–1476 (1998). [CrossRef]
  18. N. S. Makarov, M. Drobizhev, and A. Rebane, “Two-photon absorption standards in the 550–1600nm excitation wavelength range,” Opt. Express 16, 4029–4047 (2008). [CrossRef] [PubMed]
  19. See, for example, http://www.clonetech.com
  20. S. Bonsma, R. Purchase, S. Jezowski, J. Gallus, F. Könz, and S. Völker, “Green and red fluorescent proteins: photo- and thermally induced dynamics probed by site-selective spectroscopy and hole burning,” Chem. Phys. Chem. 6, 838–849 (2005). [CrossRef] [PubMed]
  21. R. Ando, H. Hama, M. Yamamoto-Hino, H. Mizuno, and A. Miyawaki, “An optical marker based in the UV-induced green-to-red photoconversion of a fluorescent protein,” Proc. Natl. Acad. Sci. USA 99, 12651–12656 (2002). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited