Immobilization of biomolecules onto surfaces according to ultraviolet light diffraction patterns
Applied Optics, Vol. 49, Issue 28, pp. 5344-5350 (2010)
http://dx.doi.org/10.1364/AO.49.005344
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Abstract
We developed a method for immobilization of biomolecules onto thiol functionalized surfaces according to UV diffraction patterns. UV light-assisted molecular immobilization proceeds through the formation of free, reactive thiol groups that can bind covalently to thiol reactive surfaces. We demonstrate that, by shaping the pattern of the UV light used to induce molecular immobilization, one can control the pattern of immobilized molecules onto the surface. Using a single-aperture spatial mask, combined with the Fourier transforming property of a focusing lens, we show that submicrometer (
© 2010 Optical Society of America
OCIS Codes
(310.1860) Thin films : Deposition and fabrication
(350.6670) Other areas of optics : Surface photochemistry
(280.1415) Remote sensing and sensors : Biological sensing and sensors
(070.6120) Fourier optics and signal processing : Spatial light modulators
ToC Category:
Fourier Optics and Signal Processing
History
Original Manuscript: July 8, 2010
Manuscript Accepted: July 15, 2010
Published: September 24, 2010
Virtual Issues
Vol. 5, Iss. 14 Virtual Journal for Biomedical Optics
Citation
Steffen Bjørn Petersen, Ane Kold di Gennaro, Maria Teresa Neves-Petersen, Esben Skovsen, and Antonietta Parracino, "Immobilization of biomolecules onto surfaces according to ultraviolet light diffraction patterns," Appl. Opt. 49, 5344-5350 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-49-28-5344
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