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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 2 — Feb. 1, 2012

Thermal diffusivity imaging with the thermal lens microscope

Oluwatosin O. Dada, Peter E. Feist, and Norman J. Dovichi  »View Author Affiliations

Applied Optics, Vol. 50, Issue 34, pp. 6336-6342 (2011)

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A coaxial thermal lens microscope was used to generate images based on both the absorbance and thermal diffusivity of histological samples. A pump beam was modulated at frequencies ranging from 50 kHz to 5 MHz using an acousto-optic modulator. The pump and a CW probe beam were combined with a dichroic mirror, directed into an inverted microscope, and focused onto the specimen. The change in the transmitted probe beam’s center intensity was detected with a photodiode. The photodiode’s signal and a reference signal from the modulator were sent to a high-speed lock-in amplifier. The in-phase and quadrature signals were recorded as a sample was translated through the focused beams and used to generate images based on the amplitude and phase of the lock-in amplifier’s signal. The amplitude is related to the absorbance and the phase is related to the thermal diffusivity of the sample. Thin sections of stained liver and bone tissues were imaged; the contrast and signal-to-noise ratio of the phase image was highest at frequencies from 0.1 1 MHz and dropped at higher frequencies. The spatial resolution was 2.5 μm for both amplitude and phase images, limited by the pump beam spot size.

© 2011 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy
(190.4870) Nonlinear optics : Photothermal effects

ToC Category:

Original Manuscript: July 25, 2011
Revised Manuscript: August 26, 2011
Manuscript Accepted: August 28, 2011
Published: November 22, 2011

Virtual Issues
Vol. 7, Iss. 2 Virtual Journal for Biomedical Optics

Oluwatosin O. Dada, Peter E. Feist, and Norman J. Dovichi, "Thermal diffusivity imaging with the thermal lens microscope," Appl. Opt. 50, 6336-6342 (2011)

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  1. J. R. Whinnery, “Laser measurement of optical-absorption in liquids,” Acc. Chem. Res. 7, 225–231 (1974). [CrossRef]
  2. N. J. Dovichi, “Thermo-optical spectrophotometries in analytical chemistry,” CRC Crit. Rev. Anal. Chem. 17, 357–424(1987). [CrossRef]
  3. S. E. Bialkowski, Photothermal Spectroscopic Method for Chemical Analysis (Wiley1996).
  4. N. J. Dovichi and J. M. Harris, “Laser-induced thermal lens effect for calorimetric trace analysis,” Anal. Chem. 53, 106–109 (1981). [CrossRef]
  5. T. G. Nolan, B. K. Hart, and N. J. Dovichi, “Photothermal refraction as a microbore liquid-chromatography detector in femtomole amino-acid determination,” Anal. Chem. 57, 2703–2705 (1985). [CrossRef]
  6. S. Hiki, K. Mawatari, A. Hibara, M. Tokeshi, and T. Kitamori, “UV excitation thermal lens microscope for sensitive and nonlabeled detection of nonfluorescent molecules,” Anal. Chem. 78, 2859–2863 (2006). [CrossRef] [PubMed]
  7. D. Boyer, P. Tamarat, A. Maali, B. Lounis, and M. Orrit, “Photothermal imaging of nanometer-sized metal particles among scatterers,” Science 297, 1160–1163 (2002). [CrossRef] [PubMed]
  8. W. A. Weimer and N. J. Dovichi, “Simple-model for the time-dependence of the periodically excited crossed-beam thermal lens,” J. Appl. Phys. 59, 225–230 (1986). [CrossRef]
  9. T. I. Chen and M. D. Morris, “Photothermal deflection densitometer for thin-layer chromatography,” Anal. Chem. 56, 19–21 (1984). [CrossRef]
  10. D. S. Burgi, T. G. Nolan, J. A. Risfelt, and N. J. Dovichi, “Photothermal refraction for scanning laser microscopy,” Opt. Eng. 23, 756–758 (1984).
  11. D. S. Burgi and N. J. Dovichi, “Submicrometer resolution images of absorbency and thermal-diffusivity with the photothermal microscope,” Appl. Opt. 26, 4665–4669(1987). [CrossRef] [PubMed]
  12. D. Lapotko, G. Kuchinsky, M. Potapnev, and D. Pechkovsky, “Photothermal image cytometry of human neutrophils,” Cytometry 24, 198–203 (1996). [CrossRef] [PubMed]
  13. J. Zheng, T. Odake, T. Kitamori, and T. Sawada, “Miniaturized ultrathin slab gel electrophoresis with thermal lens microscope detection and its application to fast genetic diagnosis,” Anal. Chem. 71, 5003–5008 (1999). [CrossRef] [PubMed]
  14. H. Kimura, K. Sekiguchi, T. Kitamori, T. Sawada, and M. Mukaida, “Assay of spherical cell surface molecules by thermal lens microscopy and its application to blood cell substances,” Anal. Chem. 73, 4333–4337 (2001). [CrossRef] [PubMed]
  15. E. Tamaki, K. Sato, M. Tokeshi, K. Sato, M. Aihara, and T. Kitamori, “Single-cell analysis by a scanning thermal lens microscope with a microchip: direct monitoring of cytochrome c distribution during apoptosis process,” Anal. Chem. 74, 1560–1564 (2002). [CrossRef] [PubMed]
  16. B. Bertussi, J. V. Natoli, and M. Commandré, “High-resolution photothermal microscope: a sensitive tool for the detection of isolated absorbing defects in optical coatings,” Appl. Opt. 45, 1410–1415 (2006). [CrossRef] [PubMed]
  17. S. J. Lu, W. Min, S. Chong, G. R. Holtom, and X. S. Xie, “Label-free imaging of heme proteins with two-photon excited photothermal lens microscopy,” Appl. Phys. Lett. 96, 113701(2010). [CrossRef]
  18. W. A. Weimer and N. J. Dovichi, “Time-resolved crossed-beam thermal lens measurement as a nonintrusive probe of flow velocity,” Appl. Opt. 24, 2981–2986 (1985). [CrossRef] [PubMed]
  19. R. McLaren and N. J. Dovichi, “Spatially resolved differential resistance of bulk superconductors by laser-induced heating,” J. Appl. Phys. 68, 4882–4884 (1990). [CrossRef]
  20. S. Wu and N. J. Dovichi, “Fresnel diffraction theory for steady-state thermal lens measurements in thin-films,” Appl. Phys. 67, 1170–1182 (1990). [CrossRef]
  21. J. P. Gordon, R. C. C. Leite, R. S. Moore, S. P. S. Porto, and J. R. Whinnery, “Long-transient effects in lasers with inserted liquid samples,” J. Appl. Phys. 36, 3–8 (1965). [CrossRef]
  22. F. Oberhettinger, Tabellen Zur Fourier Transformation (Springer-Verlag, 1957).
  23. D. J. Doss, J. D. Humphrey, and N. T. Wright, “Measurement of thermal diffusivity of bovine aorta subject to finite deformation,” Ann. NY Acad. Sci. 858, 88–97 (1998). [CrossRef]
  24. S. Biyikli, M. F. Modest, and R. Tarr, “Measurements of thermal properties for human femora,” J. Biomed. Mater. Res. 20, 1335–1345 (1986). [CrossRef] [PubMed]
  25. C. Haisch, “Quantitative analysis in medicine using photoacoustic tomography,” Anal. Bioanal. Chem. 393, 473–479(2009). [CrossRef]
  26. M. H. Xu and V. H. V. Wang, “Photoacoustic imaging in biomedicine,” Rev. Sci. Instrum. 77, 041101 (2006). [CrossRef]
  27. N. Tabatabaei, A. Mandelis, and B. T. Amaechi, “Thermal-wave radar: a novel subsurface imaging modality with extended depth-resolution dynamic range,” J. Biomed. Opt. 16, 071402 (2011). [CrossRef] [PubMed]

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