In this paper quantitative imaging of biological cells using high-resolution full-field optical coherence microscopy (FF-OCM) is reported. The FF-OCM was realized using a swept-source system, a Mirau interferometer, and a CCD camera (a two-dimensional detection unit). A Mirau-interferometric objective lens was used to generate the interferometric signal. The signal was analyzed by a Fourier analysis technique. Optically sectioned amplitude images and a quantitative phase map of biological cells such as onion skin and red blood cells (RBCs) are demonstrated. Further, the refractive index profile of the RBCs is also presented. For the 50× Mirau objective, the experimentally achieved axial and transverse resolution of the present system are 3.8 and 1.2 μm, respectively. The CCD provides parallel detection and measures enface images without X, Y, Z mechanical scanning.
© 2011 Optical Society of America
Original Manuscript: April 29, 2011
Revised Manuscript: July 15, 2011
Manuscript Accepted: July 24, 2011
Published: November 23, 2011
Vol. 7, Iss. 2 Virtual Journal for Biomedical Optics
Tulsi Anna, Vishal Srivastava, Dalip Singh Mehta, and Chandra Shakher, "High-resolution full-field optical coherence microscopy using a Mirau interferometer for the quantitative imaging of biological cells," Appl. Opt. 50, 6343-6351 (2011)