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Quantitative phase and refractive index measurements with point-source digital in-line holographic microscopy |
Applied Optics, Vol. 51, Issue 10, pp. 1503-1515 (2012)
http://dx.doi.org/10.1364/AO.51.001503
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Abstract
Point-source digital in-line holographic microscopy with numerical reconstruction is ideally suited for quantitative phase measurements to determine optical path lengths and to extract changes in refractive index within accuracy close to 0.001 on the submicrometer length scale. This is demonstrated with simulated holograms and with detailed measurements on a number of different micrometer-sized samples such as suspended drops, optical fibers, as well as organisms of biological interest such as E. coli bacteria, HeLa cells, and fibroblast cells.
© 2012 Optical Society of America
OCIS Codes
(090.0090) Holography : Holography
(110.0110) Imaging systems : Imaging systems
(110.2960) Imaging systems : Image analysis
(090.1995) Holography : Digital holography
(110.5086) Imaging systems : Phase unwrapping
ToC Category:
Imaging Systems
History
Original Manuscript: September 23, 2011
Revised Manuscript: November 14, 2011
Manuscript Accepted: November 15, 2011
Published: March 28, 2012
Virtual Issues
Vol. 7, Iss. 6 Virtual Journal for Biomedical Optics
Citation
M. H. Jericho, H. J. Kreuzer, M. Kanka, and R. Riesenberg, "Quantitative phase and refractive index measurements with point-source digital in-line holographic microscopy," Appl. Opt. 51, 1503-1515 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-51-10-1503
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