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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 6 — May. 25, 2012

Quantitative phase and refractive index measurements with point-source digital in-line holographic microscopy

M. H. Jericho, H. J. Kreuzer, M. Kanka, and R. Riesenberg  »View Author Affiliations

Applied Optics, Vol. 51, Issue 10, pp. 1503-1515 (2012)

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Point-source digital in-line holographic microscopy with numerical reconstruction is ideally suited for quantitative phase measurements to determine optical path lengths and to extract changes in refractive index within accuracy close to 0.001 on the submicrometer length scale. This is demonstrated with simulated holograms and with detailed measurements on a number of different micrometer-sized samples such as suspended drops, optical fibers, as well as organisms of biological interest such as E. coli bacteria, HeLa cells, and fibroblast cells.

© 2012 Optical Society of America

OCIS Codes
(090.0090) Holography : Holography
(110.0110) Imaging systems : Imaging systems
(110.2960) Imaging systems : Image analysis
(090.1995) Holography : Digital holography
(110.5086) Imaging systems : Phase unwrapping

ToC Category:
Imaging Systems

Original Manuscript: September 23, 2011
Revised Manuscript: November 14, 2011
Manuscript Accepted: November 15, 2011
Published: March 28, 2012

Virtual Issues
Vol. 7, Iss. 6 Virtual Journal for Biomedical Optics

M. H. Jericho, H. J. Kreuzer, M. Kanka, and R. Riesenberg, "Quantitative phase and refractive index measurements with point-source digital in-line holographic microscopy," Appl. Opt. 51, 1503-1515 (2012)

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