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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 11 — Oct. 31, 2012

Modeling of fiber-optic fluorescence probes for strongly absorbing samples

Dorit Munzke, John Saunders, Hengameh Omrani, Oliver Reich, and Hans-Peter Loock  »View Author Affiliations


Applied Optics, Vol. 51, Issue 26, pp. 6343-6351 (2012)
http://dx.doi.org/10.1364/AO.51.006343


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Abstract

The dynamic range of fiber-optic fluorescent probes such as single fibers and fiber bundles is calculated for strongly absorbing samples, such as process liquids, foodstuffs, and lubricants. The model assumes an excitation beam profile based on a Lambertian light source and uses analytical forms of the collection efficiency, followed by an Abel transformation and numerical integration. It is found that the effect of primary absorption of the excitation light and secondary absorption of the fluorescence is profound. For fiber bundles and bifurcated fiber probes, the upper accessible concentration limit is roughly given by the absorption length of the primary and secondary absorption. Fluorescence detectors that are placed at right angles to the excitation beam axis or collinear to the beam axis are equally strongly affected by secondary absorption. A probe in which the same fiber is used for excitation and for collection of the fluorescence emerges as the fiber probe with the largest accessible concentration range.

© 2012 Optical Society of America

OCIS Codes
(060.2370) Fiber optics and optical communications : Fiber optics sensors
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(220.2740) Optical design and fabrication : Geometric optical design
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence

ToC Category:
Spectroscopy

History
Original Manuscript: June 20, 2012
Revised Manuscript: August 3, 2012
Manuscript Accepted: August 5, 2012
Published: September 7, 2012

Virtual Issues
Vol. 7, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Dorit Munzke, John Saunders, Hengameh Omrani, Oliver Reich, and Hans-Peter Loock, "Modeling of fiber-optic fluorescence probes for strongly absorbing samples," Appl. Opt. 51, 6343-6351 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-51-26-6343

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