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Modeling of fiber-optic fluorescence probes for strongly absorbing samples |
Applied Optics, Vol. 51, Issue 26, pp. 6343-6351 (2012)
http://dx.doi.org/10.1364/AO.51.006343
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Abstract
The dynamic range of fiber-optic fluorescent probes such as single fibers and fiber bundles is calculated for strongly absorbing samples, such as process liquids, foodstuffs, and lubricants. The model assumes an excitation beam profile based on a Lambertian light source and uses analytical forms of the collection efficiency, followed by an Abel transformation and numerical integration. It is found that the effect of primary absorption of the excitation light and secondary absorption of the fluorescence is profound. For fiber bundles and bifurcated fiber probes, the upper accessible concentration limit is roughly given by the absorption length of the primary and secondary absorption. Fluorescence detectors that are placed at right angles to the excitation beam axis or collinear to the beam axis are equally strongly affected by secondary absorption. A probe in which the same fiber is used for excitation and for collection of the fluorescence emerges as the fiber probe with the largest accessible concentration range.
© 2012 Optical Society of America
OCIS Codes
(060.2370) Fiber optics and optical communications : Fiber optics sensors
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(220.2740) Optical design and fabrication : Geometric optical design
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence
ToC Category:
Spectroscopy
History
Original Manuscript: June 20, 2012
Revised Manuscript: August 3, 2012
Manuscript Accepted: August 5, 2012
Published: September 7, 2012
Virtual Issues
Vol. 7, Iss. 11 Virtual Journal for Biomedical Optics
Citation
Dorit Munzke, John Saunders, Hengameh Omrani, Oliver Reich, and Hans-Peter Loock, "Modeling of fiber-optic fluorescence probes for strongly absorbing samples," Appl. Opt. 51, 6343-6351 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-51-26-6343
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