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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 1 — Feb. 4, 2013

Fast algorithm for reliability-guided phase unwrapping in digital holographic microscopy

Lihong Ma, Yong Li, Hui Wang, and Hongzhen Jin  »View Author Affiliations

Applied Optics, Vol. 51, Issue 36, pp. 8800-8807 (2012)

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A fast reliability-guided phase unwrapping algorithm, using an optimized quality map and combining it with look-up table operation, is proposed for digital holographic microscopy. First, by detecting the residues in the wrapped phase map, an intensity threshold is calculated in the normalized intensity image and the measured region is distinguished into the reliable region and the doubtful region. An optimized quality map is derived by the method in which the intensity values in the reliable region are set to 1 and those in the doubtful region remain unchanged. Then the flood fill algorithm by look-up table is implemented with the optimized quality map to retrieve true phase map. The experimental results demonstrate that not only does the proposed algorithm perform well, but also the speed is significantly faster than that of the conventional flood fill algorithm using insert sorting.

© 2012 Optical Society of America

OCIS Codes
(100.5070) Image processing : Phase retrieval
(180.6900) Microscopy : Three-dimensional microscopy
(090.1995) Holography : Digital holography
(100.5088) Image processing : Phase unwrapping

ToC Category:

Original Manuscript: August 27, 2012
Revised Manuscript: November 11, 2012
Manuscript Accepted: November 26, 2012
Published: December 20, 2012

Virtual Issues
Vol. 8, Iss. 1 Virtual Journal for Biomedical Optics

Lihong Ma, Yong Li, Hui Wang, and Hongzhen Jin, "Fast algorithm for reliability-guided phase unwrapping in digital holographic microscopy," Appl. Opt. 51, 8800-8807 (2012)

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  1. E. Cuche, F. Bevilacqua, and C. Depeursinge, “Digital holography for quantitative phase-contrast imaging,” Opt. Lett. 24, 291–293 (1999). [CrossRef]
  2. L. H. Ma, H. Wang, Y. Li, and H. Z. Jin, “Partition calculation for zero-order and conjugate image removal in digital in-line holography,” Opt. Express 20, 1805–1815 (2012). [CrossRef]
  3. M. León-Rodríguez, R. Rodríguez-Vera, J. A. Rayas, and S. Calixto, “High topographical accuracy by optical shot noise reduction in digital holographic microscopy,” J. Opt. Soc. Am. A 29, 498–506 (2012). [CrossRef]
  4. C. Furlong and R. J. Pryputniewics, “Optoelectronic characterization of shape and deformations of MEMS accelerometers used in transportation applications,” Opt. Eng. 42, 1223–1231(2003). [CrossRef]
  5. T. Kozacki, M. Józwik, and K. Liżewski, “High-numerical-aperture microlens shape measurement with digital holographic microscopy,” Opt. Lett. 36, 4419–4421 (2011). [CrossRef]
  6. W. J. Qu, C. O. Choo, Y. J. Yu, and A. Asundi, “Microlens characterization by digital holographic microscopy with physical spherical phase compensation,” Appl. Opt. 49, 6448–6454 (2010). [CrossRef]
  7. L. H. Ma, H. Wang, H. Z. Jin, and Y. Li, “Experimental study on quantitative phase imaging by digital holographic microscopy,” Zhongguo Jiguang/Chin. J. Lasers 39, 0309002 (2012). [CrossRef]
  8. M. H. Jericho, H. J. Kreuzer, M. Kanka, and R. Riesenberg, “Quantitative phase and refractive index measurements with point-source digital in-line holographic microscopy,” Appl. Opt. 51, 1503–1515 (2012). [CrossRef]
  9. B. Kemper and G. v. Bally, “Digital holographic microscopy for live cell applications and technical inspection,” Appl. Opt. 47, A52–A61 (2008). [CrossRef]
  10. P. Langehanenberg, L. Ivanova, and I. Bernhardt, “Automated three-dimensional tracking of living cells by digital holographic microscopy,” J. Biomed. Opt. 14, 014018 (2009). [CrossRef]
  11. D. C. Ghiglia and M. D. Pritt, Two-Dimensional Phase Unwrapping: Theory, Algorithm, and Software (Wiley, 1998).
  12. R. M. Goldstein, H. A. Zebker, and C. L. Werner, “Satellite radar interferometry: two-dimensional phase unwrapping,” Radio Sci. 23, 713–720 (1988). [CrossRef]
  13. Y. G. Lu, X. Z. Wang, and G. T. He, “Phase unwrapping based on branch cut placing and reliability ordering,” Opt. Eng. 44, 055601 (2005). [CrossRef]
  14. X. Su and W. Chen, “Reliability-guided phase unwrapping algorithm: a review,” Opt. Lasers Eng. 42, 245–261 (2004). [CrossRef]
  15. S. Fang, L. Meng, L. J. Wang, P. C. Yang, and M. Komori, “Quality-guided phase unwrapping algorithm based on reliability evaluation,” Appl. Opt. 50, 5446–5452 (2011). [CrossRef]
  16. S. Liu, W. Xiao, F. Pan, F. Wang, and L. Cong, “Complex-amplitude-based phase unwrapping method for digital holographic microscopy,” Opt. Lasers Eng. 50, 322–327(2012). [CrossRef]
  17. S. Fang, L. Meng, L. Wang, P. Yang, and M. Komori, “Quality-guided phase unwrapping algorithm based on reliability evaluation,” Appl. Opt. 50, 5446–5452 (2011). [CrossRef]
  18. T. J. Flynn, “Two-dimensional phase unwrapping with minimum weighted discontinuity,” J. Opt. Soc. Am. A 14, 2692–2701 (1997). [CrossRef]
  19. W. Xu, “A region growing algorithm for InSAR phase unwrapping,” IEEE Trans. Geosci. Remote Sens. 37, 124–134 (1999). [CrossRef]
  20. M. A. Schofield and Y. M. Zhu, “Fast phase unwrapping algorithm for interferometric applications,” Opt. Lett. 28, 1194–1196 (2003). [CrossRef]
  21. J. Arines, “Least-squares modal estimation of wrapped phases: application to phase unwrapping,” Appl. Opt. 42, 3373–3378 (2003). [CrossRef]
  22. Y. G. Lua, X. Z. Wang, and X. P. Zhang, “Weighted least-squares phase unwrapping algorithm based on derivative variance correlation map,” Optik 118, 62–66 (2007). [CrossRef]
  23. D. Kerr, G. H. Kaufmann, and G. E. Galizzi, “Unwrapping of interferometric phase-fringe maps by the discrete cosine transform,” Appl. Opt. 35, 810–816 (1996). [CrossRef]
  24. A. Anand and W. S. Zhou, “Fast phase-unwrapping algorithm based on a gray-scale mask and flood fill,” Appl. Opt. 37, 5416–5420 (1998). [CrossRef]
  25. Y. Li and X. Su, “Fast algorithm for reliability-guided phase unwrapping,” Opt. Electron. Eng. 32, 76–79 (2005).
  26. M. Zhao, L. Huang, Q. C. Zhang, X. Y. Su, A. Asundi, and K. M. Qian, “Quality-guided phase unwrapping technique: comparison of quality maps and guiding strategies,” Appl. Opt. 50, 6214–6224 (2011). [CrossRef]
  27. L. H. Ma, H. Wang, Y. Li, and H. Z. Jin, “Effect of system parameters on the reconstructed image quality in digital holographic microscopy,” Acta Photonica Sinica 40, 300–306 (2011). [CrossRef]
  28. P. Ferraro, S. De Nicola, A. Finizio, G. Coppola, S. Grilli, C. Magro, and G. Pierattini, “Compensation of the inherent wave front curvature in digital holographic coherent microscopy for quantitative phase-contrast imaging,” Appl. Opt. 42, 1938–1946 (2003). [CrossRef]
  29. T. Colomb, E. Cuche, F. Charriére, J. Kühn, N. Aspert, F. Montfort, P. Marquet, and C. Depeursinge, “Automatic procedure for aberration compensation in digital holographic microscopy and applications to specimen shape compensation,” Appl. Opt. 45, 851–863 (2006). [CrossRef]
  30. W. Qu, C. Choo, V. Singh, Y. Yu, and A. Asundi, “Quasi-physical phase compensation in digital holographic microscopy,” J. Opt. Soc. Am. A 26, 2005–2011 (2009). [CrossRef]

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