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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 4 — Mar. 29, 2012

Optical penetration-based silkworm pupa gender sensor structure

Sarun Sumriddetchkajorn and Chakkrit Kamtongdee  »View Author Affiliations


Applied Optics, Vol. 51, Issue 4, pp. 408-412 (2012)
http://dx.doi.org/10.1364/AO.51.000408


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Abstract

This paper proposes and experimentally demonstrates for what is believed to be the first time a highly sought-after optical structure for highly-accurate identification of the silkworm pupa gender. The key idea is to exploit a long wavelength optical beam in the red or near infrared spectrum that can effectively and safely penetrate the body of a silkworm pupa. Later on, simple image processing operations via image thresholding, blob filtering, and image inversion processes are applied in order to eliminate the unwanted image noises and at the same time highlight the gender gland. Experimental proof of concept using three 636 nm wavelength light emitting diodes, a two-dimensional web camera, an 8 bit microcontroller board, and a notebook computer shows a very high 95.6% total accuracy in identifying the gender of 45 silkworm pupae with a measured fast identification time of 96.6 ms. Other key features include low cost, low component counts, and ease of implementation and control.

© 2012 Optical Society of America

OCIS Codes
(110.2960) Imaging systems : Image analysis
(120.4630) Instrumentation, measurement, and metrology : Optical inspection
(300.0300) Spectroscopy : Spectroscopy
(280.4788) Remote sensing and sensors : Optical sensing and sensors

ToC Category:
Spectroscopy

History
Original Manuscript: July 12, 2011
Revised Manuscript: September 29, 2011
Manuscript Accepted: October 3, 2011
Published: January 24, 2012

Virtual Issues
Vol. 7, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Sarun Sumriddetchkajorn and Chakkrit Kamtongdee, "Optical penetration-based silkworm pupa gender sensor structure," Appl. Opt. 51, 408-412 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ao-51-4-408

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