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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 6 — Jun. 17, 2008

Spectrograph Based on a Single Diffractive Element for Real-Time Measurement of Circular Dichroism

Pasquale Pagliusi, Clementina Provenzano, Alfredo Mazzulla, Loris Giorgini, and Gabriella Cipparrone

Applied Spectroscopy, Vol. 62, Issue 5, pp. 465-468 (2008)


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Abstract

In this study, a novel and simple diffractive spectrographic method for real-time measurements of circular dichroism (CD) is considered from a theoretical and experimental approach. A demonstrator prototype of the CD spectrograph has been developed and its performance has been compared with a commercial phase-modulation CD spectrometer. The main element of the device is a polarization holographic grating, recorded in a thin photosensitive organic film, by two interfering opposite circularly polarized beams. A peculiarity of this grating is that the amplitude of the +1 (−1) order of diffraction is proportional to the right (left) circular polarization component of the incoming beam. Here we demonstrate that the CD spectrum of a specimen can be easily evaluated from the intensities of the diffracted beams. A white light beam passing through the specimen is diffracted from the grating and the intensities of the ± 1 orders of diffraction are measured. Due to the spectral selectivity of the grating, the CD at each wavelength can be evaluated at the same time using two linear array detectors.

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Vol. 3, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Pasquale Pagliusi, Clementina Provenzano, Alfredo Mazzulla, Loris Giorgini, and Gabriella Cipparrone, "Spectrograph Based on a Single Diffractive Element for Real-Time Measurement of Circular Dichroism," Appl. Spectrosc. 62, 465-468 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=as-62-5-465

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