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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 12 — Nov. 10, 2009

Demountable Liquid/Flow Cell for in Vivo Infrared Microspectroscopy of Biological Specimens

M. J. Nasse, S. Ratti, M. Giordano, and C. J. Hirschmugl

Applied Spectroscopy, Vol. 63, Issue 10, pp. 1181-1186 (2009)


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Abstract

We have developed a liquid/flow cell/chamber allowing infrared measurements of living biological specimens with high spatial resolution under a controlled aqueous environment. This flow chamber features sub-micrometer thick diamond windows exhibiting low spherical and chromatic aberrations. Diamond has excellent transmission properties and minimal dispersion over the entire mid-infrared and visible spectral ranges. In contrast to current commercially available infrared liquid chambers, the flow chamber has a slim profile, which accommodates high resolution/magnification microscope objectives with small working distances, down to 0.6 mm above the chamber and 6 mm below the flow chamber. We have coupled a pump to the flow chamber to provide medium exchange. As an example, we present microspectroscopic infrared maps and spectra of the freshwater green alga <i>Micrasterias</i> sp. in the new flow chamber and compare them to maps obtained with a conventional liquid chamber. Pulse-amplitude-modulated fluorescence measurements on <i>Micrasterias</i> sp. cells inside the new flow chamber have been evaluated to demonstrate the viability of the algal cells.

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Vol. 4, Iss. 12 Virtual Journal for Biomedical Optics

Citation
M. J. Nasse, S. Ratti, M. Giordano, and C. J. Hirschmugl, "Demountable Liquid/Flow Cell for in Vivo Infrared Microspectroscopy of Biological Specimens," Appl. Spectrosc. 63, 1181-1186 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=as-63-10-1181

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