OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 4 — Apr. 1, 2009

Chemical Imaging of Live Cancer Cells in the Natural Aqueous Environment

Marina K. Kuimova, K. L. Andrew Chan, and Sergei G. Kazarian

Applied Spectroscopy, Vol. 63, Issue 2, pp. 164-171 (2009)


View Full Text Article

Acrobat PDF (4634 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

Chemical imaging with Fourier transform infrared (FT-IR) spectroscopy allows the visualization of the distribution of chemical components in cells without the need for labels or added dyes. However, obtaining such images of living cells is difficult because of the strong absorption of water in the mid-infrared region. We report the use of attenuated total reflection (ATR) FT-IR spectroscopic imaging to study live human cancer cells in an aqueous environment, on a single cell level. Two complementary approaches have been used, providing flexibility with field of view and spatial resolution: (1) micro-ATR FT-IR imaging using a microscope objective with a Ge crystal, and (2) single-reflection diamond ATR-FT-IR imaging. Using both approaches, the ATR-FT-IR spectroscopic signatures allow the differentiation between several cellular organelles, e.g., the nucleus and the endoplasmic reticulum (ER). The overall cell shape can be defined by the distribution of the amide II band in the measured image, while the DNA-rich nucleus and glycogen-rich ER could be imaged using the spectral bands at 1084 cm−1 and 1023 cm−1, respectively. We also demonstrate the potential of ATR-FT-IR spectroscopic imaging for unraveling the details of the dynamics of biological processes, which are not accessible from cell ensemble studies, with high molecular specificity and satisfactory spatial resolution.

Virtual Issues
Vol. 4, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Marina K. Kuimova, K. L. Andrew Chan, and Sergei G. Kazarian, "Chemical Imaging of Live Cancer Cells in the Natural Aqueous Environment," Appl. Spectrosc. 63, 164-171 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=as-63-2-164


Sort:  Journal  |  Reset

References

References are not available for this paper.

Cited By

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited