OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 5, Iss. 14 — Nov. 16, 2010

Numerical Correction of Detector Channel Cross-Talk Using Full-Spectrum Fluorescence Correlation Spectroscopy

Carol A. Roach and Sharon L. Neal

Applied Spectroscopy, Vol. 64, Issue 10, pp. 1145-1153 (2010)


View Full Text Article

Acrobat PDF (353 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

Fluorescence correlation spectroscopy (FCS) uses fluctuations in the fluorescence collected from a small illuminated volume to measure dynamic processes of fluorophores. In traditional FCS, spectral overlap produces cross-talk in dedicated detector channels, undermining the accuracy of measurements of molecular interactions. Here, the experimental realization of full-spectrum fluorescence correlation spectroscopy is described and coupled with multivariate data analysis to numerically correct detector cross-talk, isolating spectra and fluctuation traces of mixture components in spite of overlap. Application of this methodology is illustrated using the measurement of the diffusion constant of labeled polystyrene in hydroxypropyl cellulose in the presence of a persistent dye. Additionally, the results show that full-spectrum FCS with multivariate analysis can isolate and characterize signals from unanticipated sample components.

Virtual Issues
Vol. 5, Iss. 14 Virtual Journal for Biomedical Optics

Citation
Carol A. Roach and Sharon L. Neal, "Numerical Correction of Detector Channel Cross-Talk Using Full-Spectrum Fluorescence Correlation Spectroscopy," Appl. Spectrosc. 64, 1145-1153 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=as-64-10-1145

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited