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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 6 — Jun. 17, 2008

Group refractive index reconstruction with broadband interferometric confocal microscopy

Daniel L. Marks, Simon C. Schlachter, Adam M. Zysk, and Stephen A. Boppart  »View Author Affiliations

JOSA A, Vol. 25, Issue 5, pp. 1156-1164 (2008)

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We propose a novel method of measuring the group refractive index of biological tissues at the micrometer scale. The technique utilizes a broadband confocal microscope embedded into a Mach–Zehnder interferometer, with which spectral interferograms are measured as the sample is translated through the focus of the beam. The method does not require phase unwrapping and is insensitive to vibrations in the sample and reference arms. High measurement stability is achieved because a single spectral interferogram contains all the information necessary to compute the optical path delay of the beam transmitted through the sample. Included are a physical framework defining the forward problem, linear solutions to the inverse problem, and simulated images of biologically relevant phantoms.

© 2008 Optical Society of America

OCIS Codes
(100.3010) Image processing : Image reconstruction techniques
(100.3190) Image processing : Inverse problems
(170.1790) Medical optics and biotechnology : Confocal microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Image Processing

Original Manuscript: September 28, 2007
Manuscript Accepted: December 16, 2007
Published: April 25, 2008

Virtual Issues
Vol. 3, Iss. 6 Virtual Journal for Biomedical Optics

Daniel L. Marks, Simon C. Schlachter, Adam M. Zysk, and Stephen A. Boppart, "Group refractive index reconstruction with broadband interferometric confocal microscopy," J. Opt. Soc. Am. A 25, 1156-1164 (2008)

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