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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 6 — Jun. 17, 2008

Group refractive index reconstruction with broadband interferometric confocal microscopy

Daniel L. Marks, Simon C. Schlachter, Adam M. Zysk, and Stephen A. Boppart  »View Author Affiliations


JOSA A, Vol. 25, Issue 5, pp. 1156-1164 (2008)
http://dx.doi.org/10.1364/JOSAA.25.001156


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Abstract

We propose a novel method of measuring the group refractive index of biological tissues at the micrometer scale. The technique utilizes a broadband confocal microscope embedded into a Mach–Zehnder interferometer, with which spectral interferograms are measured as the sample is translated through the focus of the beam. The method does not require phase unwrapping and is insensitive to vibrations in the sample and reference arms. High measurement stability is achieved because a single spectral interferogram contains all the information necessary to compute the optical path delay of the beam transmitted through the sample. Included are a physical framework defining the forward problem, linear solutions to the inverse problem, and simulated images of biologically relevant phantoms.

© 2008 Optical Society of America

OCIS Codes
(100.3010) Image processing : Image reconstruction techniques
(100.3190) Image processing : Inverse problems
(170.1790) Medical optics and biotechnology : Confocal microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Image Processing

History
Original Manuscript: September 28, 2007
Manuscript Accepted: December 16, 2007
Published: April 25, 2008

Virtual Issues
Vol. 3, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Daniel L. Marks, Simon C. Schlachter, Adam M. Zysk, and Stephen A. Boppart, "Group refractive index reconstruction with broadband interferometric confocal microscopy," J. Opt. Soc. Am. A 25, 1156-1164 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=josaa-25-5-1156


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References

  1. A. M. Zysk, E. J. Chaney, and S. A. Boppart, “Refractive index of carcinogen-induced rat mammary tumours,” Phys. Med. Biol. 51, 2165-2177 (2006). [CrossRef] [PubMed]
  2. L. Lepetit, G. Cheriaux, and M. Joffre, “Linear techniques of phase measurement by femtosecond spectral interferometry for applications in spectroscopy,” J. Opt. Soc. Am. B 12, 2467-2474 (1995). [CrossRef]
  3. S. Singh, “Refractive index measurement and its applications,” Phys. Scr. 65, 167-180 (2002). [CrossRef]
  4. A. M. Zysk, F. T. Nguyen, A. L. Oldenburg, D. L. Marks, and S. A. Boppart, “Optical coherence tomography: a review of clinical development from bench to bedside,” J. Biomed. Opt. 12, 051403 (2007). [CrossRef] [PubMed]
  5. S. R. Arridge, “Optical tomography in medical imaging,” Inverse Probl. 15, R41-R93 (1999). [CrossRef]
  6. Y.-P. Wang, D.-N. Wang, W. Jin, J.-P. Chen, X.-W. Li, and J.-H. Zhou, “Reflectometry measuring refractive index and thickness of polymer samples simultaneously,” J. Mod. Opt. 53, 1845-1851 (2006). [CrossRef]
  7. H. Takubo, “Refractive index as a measure for saturation and supersaturation in crystal growth of water-soluble substances,” J. Cryst. Growth 104, 239-244 (1990). [CrossRef]
  8. Z. Liu, X. Dong, Q. Chen, C. Yin, Y. Xu, and Y. Zheng, “Nondestructive measurement of an optical fiber refractive-index profile by a transmitted-light differential interference contact microscope,” Appl. Opt. 43, 1485-1492 (2004). [CrossRef] [PubMed]
  9. D. J. Faber, M. C. G. Aalders, E. G. Mik, B. A. Hooper, M. J. C. van Gemert, and T. G. van Leeuwen, “Oxygen saturation-dependent absorption and scattering of blood,” Phys. Rev. Lett. 93, 028102 (2004). [CrossRef] [PubMed]
  10. G. J. Tearney, M. E. Brezinski, J. F. Southern, B. E. Bouma, M. R. Hee, and J. G. Fujimoto, “Determination of the refractive index of highly scattering human tissue by optical coherence tomography,” Opt. Lett. 20, 2258-2260 (1995). [CrossRef] [PubMed]
  11. A. M. Zysk, J. J. Reynolds, D. L. Marks, P. S. Carney, and S. A. Boppart, “Projected index computed tomography,” Opt. Lett. 28, 701-703 (2003). [CrossRef] [PubMed]
  12. S. A. Alexandrov, A. V. Zvyagin, K. K. M. B. D. Silva, and D. D. Sampson, “Bifocal optical coherence refractometry of turbid media,” Opt. Lett. 28, 117-119 (2003). [CrossRef] [PubMed]
  13. A. V. Zvyagin, K. K. M. B. D. Silva, S. A. Alexandrov, T. R. Hillman, J. J. Armstrong, T. Tsuzuki, and D. D. Sampson, “Refractive index tomography of turbid media by bifocal optical coherence refractometry,” Opt. Express 11, 3503-3517 (2003). [CrossRef] [PubMed]
  14. W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, “Tomographic phase microscopy,” Nat. Methods 4, 717-719 (2007). [CrossRef] [PubMed]
  15. J. J. J. Dirckx, L. C. Kuypers, and W. F. Decraemer, “Refractive index of tissue measured with confocal microscopy,” J. Biomed. Opt. 10, 044014 (2005). [CrossRef]
  16. I. K. Ilev, R. W. Waynant, K. R. Byrnes, and J. J. Anders, “Dual confocal fiber-optic method for measurement of refractive index and thickness of optically transparent media,” Opt. Lett. 27, 1603-1605 (2002). [CrossRef]
  17. D. F. Aldridge, “Linearization of the eikonal equation,” Geophysics 59, 1631-1632 (1994). [CrossRef]
  18. R. Snieder and D. F. Aldridge, “Perturbation theory for travel times,” J. Acoust. Soc. Am. 98, 1565-1569 (1995). [CrossRef]
  19. M. Born and E. Wolf, Principles of Optics (Cambridge U. Press, 1980).
  20. G. Gbur and E. Wolf, “Relation between computed tomography and diffraction tomography,” J. Opt. Soc. Am. A 18, 2132-2137 (2001). [CrossRef]
  21. G. H. Golub and C. F. Van Loan, Matrix Computations (Johns Hopkins U. Press, 1996).
  22. J. Fingler, D. Schwartz, C. Yang, and S. E. Fraser, “Mobility and transverse flow visualization using phase variance contrast with spectral optical coherence tomography,” Opt. Express 15, 12636-12653 (2007). [CrossRef] [PubMed]
  23. B. H. Park, M. C. Pierce, B. Cense, S.-H. Yun, M. Mujat, G. J. Tearney, B. E. Bouma, and J. F. de Boer, “Real-time fiber-based multi-functional spectral-domain optical coherence tomography at 1.3 microns,” Opt. Express 13, 3931-3944 (2005). [CrossRef] [PubMed]

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