Femtosecond pulse characterization and compensation using multiphoton intrapulse interference phase scan (MIIPS) [ Opt. Lett. 29, 775 (2004) ] was rigorously tested. MIIPS was found to have 3 mrad precision within the 90 nm bandwidth of the pulses. Group-velocity dispersion measurements of glass and quartz provided independent accuracy tests. Phase distortions from high-numerical-aperture objectives were measured and corrected using MIIPS, an important requirement for reproducible two-photon microscopy. Phase compensation greatly improved the pulse-shaping results through a more accurate delivery of continuous and binary phase functions to the sample. MIIPS measurements were possible through the scattering of biological tissue, a consideration for biomedical imaging.

© 2006 Optical Society of America

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