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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 6 — Jun. 13, 2006

In vivo nonlinear spectral imaging in mouse skin

J. A. Palero, H. S. de Bruijn, A. van der Ploeg-van den Heuvel, H. J. C. M. Sterenborg, and H. C. Gerritsen  »View Author Affiliations

Optics Express, Vol. 14, Issue 10, pp. 4395-4402 (2006)

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We report on two-photon autofluorescence and second harmonic spectral imaging of live mouse tissues. The use of a high sensitivity detector and ultraviolet optics allowed us to record razor-sharp deep-tissue spectral images of weak autofluorescence and short-wavelength second harmonic generation by mouse skin. Real-color image representation combined with depth-resolved spectral analysis enabled us to identify tissue structures. The results show that linking nonlinear deep-tissue imaging microscopy with autofluorescence spectroscopy has the potential to provide important information for the diagnosis of skin tissues.

© 2006 Optical Society of America

OCIS Codes
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(180.2520) Microscopy : Fluorescence microscopy
(190.4160) Nonlinear optics : Multiharmonic generation
(190.4180) Nonlinear optics : Multiphoton processes

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: February 9, 2006
Revised Manuscript: April 25, 2006
Manuscript Accepted: April 26, 2006
Published: May 15, 2006

Virtual Issues
Vol. 1, Iss. 6 Virtual Journal for Biomedical Optics

Jonathan A. Palero, H. S. de Bruijn, A. van der Ploeg-van den Heuvel, H. J. C. M. Sterenborg, and H. C. Gerritsen, "In vivo nonlinear spectral imaging in mouse skin," Opt. Express 14, 4395-4402 (2006)

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