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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 5 — May. 5, 2006

Three-dimensional optical force field on a Chinese hamster ovary cell in a fiber-optical dual-beam trap

Ming-Tzo Wei, Kun-Ta Yang, Artahses Karmenyan, and Arthur Chiou  »View Author Affiliations

Optics Express, Vol. 14, Issue 7, pp. 3056-3064 (2006)

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We used a fiber-optical dual-beam trap (single-mode fiber, λ = 532nm, trapping power ∼ 22mW, the distance between the two fiber end-faces = 125μm) to capture a Chinese hamster ovary (CHO) cell with a diameter of approximately 15μm and tracked its three-dimensional Brownian motion via a pair of orthogonal quadrant photodiodes. By analyzing the Brownian motion of the trapped CHO cell, we determined the force constants of the optical force field on the CHO cell to be kx=6.75 pN/μm, ky=5.53 pN/μm, kz=1.96 pN/μm, and kx=2.91 pN/μm, ky=2.7 pN/μm, kz=0.79 pN/μm, respectively, before and after the CHO cell was treated with latrunculin, a toxic drug known to disrupt the cytoskeleton of the cell.

© 2006 Optical Society of America

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.1420) Medical optics and biotechnology : Biology
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation

ToC Category:

Original Manuscript: January 9, 2006
Revised Manuscript: March 16, 2006
Manuscript Accepted: March 18, 2006
Published: April 3, 2006

Virtual Issues
Vol. 1, Iss. 5 Virtual Journal for Biomedical Optics

Ming-Tzo Wei, Kun-Ta Yang, Artahses Karmenyan, and Arthur Chiou, "Three-dimensional optical force field on a Chinese hamster ovary cell in a fiber-optical dual-beam trap," Opt. Express 14, 3056-3064 (2006)

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