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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 5 — May. 5, 2006

One-laser interferometric broadband coherent anti-Stokes Raman scattering

Tak W. Kee, Hongxia Zhao, and Marcus T. Cicerone  »View Author Affiliations

Optics Express, Vol. 14, Issue 8, pp. 3631-3640 (2006)

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We introduce an interferometric technique for eliminating the non-resonant background of broadband coherent anti-Stokes Raman scattering (CARS) microscopy. CARS microscopy has been used for imaging a number of biological samples and processes, but the studies are mostly limited to detecting lipids in biological systems by probing the C-H stretch. Non-resonant background and incoherent noise sources can easily overwhelm less intense signals from other molecular vibrations. In this study, we demonstrate a one-laser broadband interferometric technique that separates the spontaneous Raman scattering-related component of the CARS signal from the non-resonant background using liquid benzonitrile as a model system.

© 2006 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(190.4370) Nonlinear optics : Nonlinear optics, fibers
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering

ToC Category:

Original Manuscript: February 17, 2006
Revised Manuscript: March 28, 2006
Manuscript Accepted: March 30, 2006
Published: April 17, 2006

Virtual Issues
Vol. 1, Iss. 5 Virtual Journal for Biomedical Optics

Tak W. Kee, Hongxia Zhao, and Marcus T. Cicerone, "One-laser interferometric broadband coherent anti-Stokes Raman scattering," Opt. Express 14, 3631-3640 (2006)

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  24. Certain equipment, instruments or materials are identified in this paper in order to adequately specify the experimental details. Such identification does not imply recommendation by the National Institute of Standards and Technology nor does it imply the materials are necessarily the best available for the purpose.

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