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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 9 — Sep. 26, 2007

Multidimensional non-linear laser imaging of Basal Cell Carcinoma

R. Cicchi, D. Massi, S. Sestini, P. Carli, V. De Giorgi, T. Lotti, and F. S. Pavone  »View Author Affiliations

Optics Express, Vol. 15, Issue 16, pp. 10135-10148 (2007)

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We have used a multidimensional non-linear laser imaging approach to visualize ex-vivo samples of basal cell carcinoma (BCC). A combination of several non-linear laser imaging techniques involving fluorescence lifetime, multispectral two-photon and second-harmonic generation imaging has been used to image different skin layers. This approach has elucidated some morphological (supported by histopathological images), biochemical, and physiochemical differences of the healthy samples with respect to BCC ones. In particular, in comparison with normal skin, BCC showed a blue-shifted fluorescence emission, a higher fluorescence response at 800 nm excitation wavelength and a slightly longer mean fluorescence lifetime. Finally, the use of aminolevulinic acid as a contrast agent has been demonstrated to increase the constrast in tumor border detection. The results obtained provide further support for in-vivo non-invasive imaging of Basal Cell Carcinoma.

© 2007 Optical Society of America

OCIS Codes
(110.4190) Imaging systems : Multiple imaging
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(180.5810) Microscopy : Scanning microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: July 17, 2007
Revised Manuscript: July 24, 2007
Manuscript Accepted: July 24, 2007
Published: July 26, 2007

Virtual Issues
Vol. 2, Iss. 9 Virtual Journal for Biomedical Optics

R. Cicchi, D. Massi, S. Sestini, P. Carli, V. De Giorgi, T. Lotti, and F. S. Pavone, "Multidimensional non-linear laser imaging of Basal Cell Carcinoma," Opt. Express 15, 10135-10148 (2007)

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