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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 3 — Mar. 7, 2007

Phase-sensitive time-modulated surface plasmon resonance polarimetry for wide dynamic range biosensing

P. P. Markowicz, W. C. Law, A. Baev, P. N. Prasad, S. Patskovsky, and A. V. Kabashin  »View Author Affiliations

Optics Express, Vol. 15, Issue 4, pp. 1745-1754 (2007)

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A novel polarimetry scheme is proposed to improve the performance of phase-sensitive Surface Plasmon Resonance (SPR) biosensors. The scheme uses s-polarized light, not affected by SPR, as a reference beam, while information on the phase of the p-polarized component is obtained from an analysis of phase-polarization state of light of mixed polarization. We utilize temporal modulation of the beam reflected under SPR by a photo-elastic modulator and show that, under certain birefringent geometry, the signals at the 2nd and 3rd harmonics of modulated frequency can provide ultra-sensitive phase-based response to changes of the refractive index (thickness) of thin films on gold. We also show that the proposed configuration significantly improves detection limit compared to conventional intensity-sensitive SPR, yet enables to maintain wide dynamic range of measurements, which is normally difficult with phase-sensitive SPR schemes. Biosensing applications of the proposed scheme are illustrated in a biological model reaction of avidin – biotin binding on gold.

© 2007 Optical Society of America

OCIS Codes
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(240.6680) Optics at surfaces : Surface plasmons

ToC Category:
Optics at Surfaces

Original Manuscript: October 27, 2006
Revised Manuscript: February 2, 2007
Manuscript Accepted: February 3, 2007
Published: February 19, 2007

Virtual Issues
Vol. 2, Iss. 3 Virtual Journal for Biomedical Optics

P. P. Markowicz, W. C. Law, A. Baev, P. N. Prasad, S. Patskovsky, and A. Kabashin, "Phase-sensitive time-modulated surface plasmon resonance polarimetry for wide dynamic range biosensing," Opt. Express 15, 1745-1754 (2007)

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