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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 6 — Jun. 17, 2008

Molecular interferometric imaging

Ming Zhao, Xuefeng Wang, and David D. Nolte  »View Author Affiliations

Optics Express, Vol. 16, Issue 10, pp. 7102-7118 (2008)

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Common-path in-line shearing interferometry, combined with pixel-array imaging, provides a surface metrology that achieves 15 pm surface height resolution. An eighth-wave thermal oxide on silicon generates a reference wave locked in the condition of phase quadrature for phase-to-intensity conversion that makes surface height or index variations directly detectable by an imaging system. The scaling surface mass sensitivity for the surface metrology application is Sscal=7 fg/mm under 40× magnification with a molecular resolution of approximately 12 IgG molecules within a pixel, limited by the surface roughness of the substrate. When applied to reverse-phase immunoassays in an antibody microarray format under 7× magnification, the current limit of detection is 10 ng/ml for 1 hour incubation, limited by biological and chemical variability. The biosensor is compatible with real-time binding measurements under active flow conditions with a binding dynamic range per well of 103 and a mass sensitivity of 2 pg/mm2.

© 2008 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.0180) Medical optics and biotechnology : Microscopy
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(110.3175) Imaging systems : Interferometric imaging

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: September 7, 2007
Revised Manuscript: December 4, 2007
Manuscript Accepted: April 29, 2008
Published: May 2, 2008

Virtual Issues
Vol. 3, Iss. 6 Virtual Journal for Biomedical Optics

Ming Zhao, Xuefeng Wang, and David D. Nolte, "Molecular interferometric imaging," Opt. Express 16, 7102-7118 (2008)

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