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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 6 — Jun. 17, 2008

Achieving cellular resolution for in vivo retinal images of transgenic GFAP–GFP mice via image processing

S. Kumar, G. Ho, K.M. Woo, and L. Zhuo  »View Author Affiliations


Optics Express, Vol. 16, Issue 11, pp. 8250-8262 (2008)
http://dx.doi.org/10.1364/OE.16.008250


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Abstract

In vivo retinal images of transgenic mice, expressing GFP under the control of the GFAP (glial fibrillary acidic protein) promoter, have very poor signal-to-noise ratio (SNR) and cellular resolution such that the analysis of GFAP–GFP expressing retinal cells from these images can be a very challenging task. We report an image averaging method based on a pixel rank matching criterion which significantly enhances both these image attributes. We also show that it compares favorably against direct image averaging and a commercial averaging routine available from the Heidelberg Retinal Angiograph 2 software.

© 2008 Optical Society of America

OCIS Codes
(100.1830) Image processing : Deconvolution
(100.2000) Image processing : Digital image processing
(110.4280) Imaging systems : Noise in imaging systems
(330.2210) Vision, color, and visual optics : Vision - eye movements
(330.4300) Vision, color, and visual optics : Vision system - noninvasive assessment
(330.4460) Vision, color, and visual optics : Ophthalmic optics and devices

ToC Category:
Image Processing

History
Original Manuscript: January 15, 2008
Revised Manuscript: May 11, 2008
Manuscript Accepted: May 14, 2008
Published: May 22, 2008

Virtual Issues
Vol. 3, Iss. 6 Virtual Journal for Biomedical Optics

Citation
S. Kumar, G. Ho, K. M. Woo, and L. Zhuo, "Achieving cellular resolution for in vivo retinal images of transgenic GFAP-GFP mice via image processing," Opt. Express 16, 8250-8262 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-16-11-8250


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