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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 10 — Sep. 22, 2008

Pseudo-random single photon counting for time-resolved optical measurement

Qiang Zhang, Hock Wei Soon, Haiting Tian, Shakith Fernando, Yajun Ha, and Nan Guang Chen  »View Author Affiliations

Optics Express, Vol. 16, Issue 17, pp. 13233-13239 (2008)

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We report a new time-resolved optical measurement method which combines single photon counting and the spread spectrum time-resolved optical measurement method. A laser diode modulated with pseudo-random bit sequences replaces the short pulse laser used in conventional time-resolved optical systems, while a single photon detector records the pulse sequence in response to the modulated excitation. Periodic cross-correlation is used to retrieve the impulse response. Feasibility of our approach is validated experimentally. A rise time around 150 picoseconds has been achieved with our prototype. Besides high temporal resolution, the new method also affords other benefits such as high photon counting rate, fast data acquisition, portability, and low cost.

© 2008 Optical Society of America

OCIS Codes
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(300.6500) Spectroscopy : Spectroscopy, time-resolved

ToC Category:
Instrumentation, Measurement, and Metrology

Original Manuscript: June 19, 2008
Revised Manuscript: August 8, 2008
Manuscript Accepted: August 10, 2008
Published: August 13, 2008

Virtual Issues
Vol. 3, Iss. 10 Virtual Journal for Biomedical Optics

Qiang Zhang, Hock Wei Soon, Haiting Tian, Shakith Fernando, Yajun Ha, and Nan Guang Chen, "Pseudo-random single photon counting for time-resolved optical measurement," Opt. Express 16, 13233-13239 (2008)

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  1. M. S. Patterson, B. Chance, and B. C. Wilson, "Time resolved reflectance and transmittance for the noninvasive measurement of tissue optical properties," Appl. Opt. 28, 2331-2336 (1989). [CrossRef] [PubMed]
  2. J. R. Lakowicz, H. Szmacinski, K. Nowaczyk, K.W. Berndt, and M. Johnson, "Fluorescence lifetime imaging," Anal. Biochem. 202, 316-330 (1992). [CrossRef] [PubMed]
  3. P. I. H. Bastiaens and A. Squire, "Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell," Trends Cell Biol. 9, 48-52 (1999). [CrossRef] [PubMed]
  4. F. Gao, H. J. Zhao, and Y. Yamada, "Improvement of Image Quality in Diffuse Optical Tomography by use of Full Time-Resolved Data," Appl. Opt. 41, 778-791 (2002). [CrossRef] [PubMed]
  5. M. Essenpreis, C. E. Elwell, M. Cope, P. van der Zee, S. R. Arridge, and D. T. Delpy, "Spectral dependence of temporal point spread functions in human tissues," Appl. Opt. 32, 418-425 (1993). [CrossRef] [PubMed]
  6. W. Becker, A. Bergmann, M. A. Hink, K. König, K. Benndorf, and C. Biskup, "Fluorescence lifetime imaging by time-correlated single-photon counting," Microsc. Res. Tech. 63, 58-66 (2004). [CrossRef]
  7. D. V. O'Connor and D. Phillips, "Basic Principle of the Single Photon Counting Lifetime Measurement," in Time-correlated single photon counting, (Academic, 1984), pp. 36-54.
  8. Becker & Hickl GmbH, "The bh TCSPC handbook,"http://www.becker-hickl.com/literature.htm.
  9. D. Grosenick, H. Wabnitz, H. H. Rinneberg, K. T. Moesta, and P. M. Schlag, "Development of a Time-Domain Optical Mammograph and First in vivo Applications," Appl. Opt. 38, 2927-2943 (1999). [CrossRef]
  10. R. Cubeddu, A. Pifferi, P. Taroni, A. Torricelli, and G. Valentini, "Compact Tissue Oximeter Based on Dual-Wavelength Multichannel Time-Resolved Reflectance," Appl. Opt. 38, 3670-3680 (1999). [CrossRef]
  11. M. Prummer, C. G. Hübner, B. Sick, B. Hecht, A. Renn, and U. P. Wild, "Single-Molecule Identification by Spectrally and Time-Resolved Fluorescence Detection," Anal. Chem. 72, 443 - 447 (2000). [CrossRef] [PubMed]
  12. P. Schwille, S. Kummer, A. A. Heikal, W. E. Moerner, and W. W. Webb, "Fluorescence correlation spectroscopy reveals fast optical excitation-driven intramolecular dynamics of yellow fluorescent proteins," PNAS,  97, 151-156 (2000) [CrossRef] [PubMed]
  13. H. Eda, I. Oda, Y. Ito, Y. Wada, Y. Oikawa, Y. Tsunazawa, and M. Takada, "Multichannel time-resolved optical tomographic imaging system," Rev. Sci. Instrum. 70, 3595-3602 (1999). [CrossRef]
  14. F. E. W. Schmidt, M. E. Fry, E. M. C. Hillman, J. C. Hebden, and D. T. Delpy, "A 32-channel time-resolved instrument for medical optical tomography," Rev. Sci. Instrum. 71, 256-265 (2000). [CrossRef]
  15. N. G. Chen and Q. Zhu, "Time-resolved diffusive optical tomography using pseudo-random sequences," Opt. Express. 11, 3445-3454 (2003). [CrossRef] [PubMed]
  16. N. G. Chen and Q. Zhu, "Time-resolved optical measurements with spread spectrum excitations," Opt. Lett. 27, 1806-1808 (2002). [CrossRef]
  17. N. G. Chen and Q. Zhu, "Spread spectrum time-resolved photon migration imaging system: the principle and simulation results," Proc. SPIE 4955, 474-479 (2003). [CrossRef]

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