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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 5 — May. 5, 2009

Volumetric retinal imaging with ultrahigh-resolution spectral-domain optical coherence tomography and adaptive optics using two broadband light sources

Barry Cense, Eric Koperda, Jeffrey M. Brown, Omer P. Kocaoglu, Weihua Gao, Ravi S. Jonnal, and Donald T. Miller  »View Author Affiliations

Optics Express, Vol. 17, Issue 5, pp. 4095-4111 (2009)

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Ultrabroadband sources, such as multiplexed superluminescent diodes (SLDs) and femtosecond lasers, have been successfully employed in adaptive optics optical coherence tomography (AO-OCT) systems for ultrahigh resolution retinal imaging. The large cost differential of these sources, however, motivates the need for a performance comparison. Here, we compare the performance of a Femtolasers Integral Ti:Sapphire laser and a Superlum BroadLighter T840, using the same AO-OCT system and the same subject. In addition, we investigate the capability of our instrument equipped with the Integral to capture volume images of the fovea and adjacent regions on a second subject using the AO to control focus in the retina and custom and freeware image registration software to reduce eye motion artifacts. Monochromatic ocular aberrations were corrected with a woofer-tweeter AO system. Coherence lengths of the Integral and BroadLighter were measured in vivo at 3.2 µm and 3.3 µm, respectively. The difference in dynamic range was 5 dB, close to the expected variability of the experiment. Individual cone photoreceptors, retinal capillaries and nerve fiber bundles were distinguished in all three dimensions with both sources. The acquired retinal volumes are provided for viewing in OSA ISP, allowing the reader to data mine at the microscope level.

© 2009 Optical Society of America

OCIS Codes
(010.1080) Atmospheric and oceanic optics : Active or adaptive optics
(170.4470) Medical optics and biotechnology : Ophthalmology
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(330.4300) Vision, color, and visual optics : Vision system - noninvasive assessment

ToC Category:
OCT Technology

Original Manuscript: October 8, 2008
Revised Manuscript: January 7, 2009
Manuscript Accepted: January 19, 2009
Published: March 2, 2009

Virtual Issues
Vol. 4, Iss. 5 Virtual Journal for Biomedical Optics
Interactive Science Publishing Focus Issue: Optical Coherence Tomography (OCT) (2009) Optics Express

Barry Cense, Erik Koperda, Jeffrey M. Brown, Omer P. Kocaoglu, Weihua Gao, Ravi S. Jonnal, and Donald T. Miller, "Volumetric retinal imaging with ultrahigh-resolution spectral-domain optical coherence tomography and adaptive optics using two broadband light sources," Opt. Express 17, 4095-4111 (2009)

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