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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 2 — Jan. 21, 2010

Real time imaging of femtosecond laser induced nano-neurosurgery dynamics in C. elegans

Susana I. C. O. Santos, Manoj Mathew, and Pablo Loza-Alvarez  »View Author Affiliations

Optics Express, Vol. 18, Issue 1, pp. 364-377 (2010)

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In this study we present for the first time the use of confocal microscopy and laser scanning brightfield microscopy (LSBF) for real time imaging of femtosecond laser nanosurgery and its dynamics in C. elegans. A single multimodal optical workstation that provides the ability to perform femtosecond laser nanosurgery and simultaneous confocal and LSBF imaging was used for the purpose. With this tool several dynamic phenomena concomitant with laser nanosurgery in C. elegans were observed and imaged. Some of these dynamic phenomena, like muscular contraction and single muscle cell stimulation, have been imaged for the first time during nano-neurosurgery of C. elegans.

© 2009 OSA

OCIS Codes
(170.1020) Medical optics and biotechnology : Ablation of tissue
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: November 3, 2009
Revised Manuscript: December 18, 2009
Manuscript Accepted: December 18, 2009
Published: December 24, 2009

Virtual Issues
Vol. 5, Iss. 2 Virtual Journal for Biomedical Optics

Susana I. C. O. Santos, Manoj Mathew, and Pablo Loza-Alvarez, "Real time imaging of femtosecond laser induced nano-neurosurgery dynamics in C. elegans," Opt. Express 18, 364-377 (2010)

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  1. N. Shen, D. Datta, C. B. Schaffer, P. LeDuc, D. E. Ingber, and E. Mazur, “Ablation of cytoskeletal filaments and mitochondria in live cells using a femtosecond laser nanoscissor,” Mech. Chem. Biosyst. 2(1), 17–25 (2005). [PubMed]
  2. H. Hirase, V. Nikolenko, J. H. Goldberg, and R. Yuste, “Multiphoton Stimulation of Neurons Multiphoton stimulation of neurons,” Neurobiol. 51(3), 237–247 (2002). [CrossRef]
  3. R. L. Amy and R. Storb, “Selective mitochondrial damage by a ruby laser microbeam: an electron microscopic study,” Science 150(3697), 756–758 (1965). [CrossRef] [PubMed]
  4. M. F. Yanik, H. Cinar, H. N. Cinar, A. D. Chisholm, Y. Jin, and A. Ben-Yakar, “Neurosurgery: functional regeneration after laser axotomy,” Nature 432(7019), 822 (2004). [CrossRef] [PubMed]
  5. R. R. Gattass, L. R. Cerami, and E. Mazur, “Micromachining of bulk glass with bursts of femtosecond laser pulses at variable repetition rates,” Opt. Express 14(12), 5279–5284 (2006), http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-14-12-5279 . [CrossRef] [PubMed]
  6. S. M. Eaton, H. B. Zhang, P. R. Herman, F. Yoshino, L. Shah, J. Bovatsek, and A. Arai, “Heat accumulation effects in femtosecond laser-written waveguides with variable repetition rate,” Opt. Express 13(12), 4708–4716 (2005), http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-13-12-4708 . [CrossRef] [PubMed]
  7. Z. Wu, A. Ghosh-Roy, M. F. Yanik, J. Z. Zhang, Y. Jin, and A. D. Chisholm, “Caenorhabditis elegans neuronal regeneration is influenced by life stage, ephrin signaling, and synaptic branching,” Proc. Natl. Acad. Sci. U.S.A. 104(38), 15132–15137 (2007). [CrossRef] [PubMed]
  8. S. Psilodimitrakopoulos, S. I. C. O. Santos, I. Amat-Roldan, A. K. N. Thayil, D. Artigas, and P. Loza-Alvarez, “In vivo, pixel-resolution mapping of thick filaments’ orientation in nonfibrilar muscle using polarization-sensitive second harmonic generation microscopy,” J. Biomed. Opt. 14(1), 014001 (2009). [CrossRef] [PubMed]
  9. P. J. Campagnola and L. M. Loew, “Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms,” Nat. Biotechnol. 21(11), 1356–1360 (2003). [CrossRef] [PubMed]
  10. W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248(4951), 73–76 (1990). [CrossRef] [PubMed]
  11. J. Squier, M. Muller, G. Brakenhoff, and K. R. Wilson, “Third harmonic generation microscopy,” Opt. Express 3(9), 315–324 (1998), http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-3-9-315 . [CrossRef] [PubMed]
  12. D. Yelin and Y. Silberberg, “Laser scanning third-harmonic-generation microscopy in biology,” Opt. Express 5(8), 169–175 (1999), http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-5-8-169 . [CrossRef] [PubMed]
  13. E. J. Gualda, G. Filippidis, G. Voglis, M. Mari, C. Fotakis, and N. Tavernarakis, “In vivo imaging of cellular structures in Caenorhabditis elegans by combined TPEF, SHG and THG microscopy,” J. Microsc. 229(1), 141–150 (2008). [CrossRef] [PubMed]
  14. E. J. Gualda, G. Filippidis, M. Mari, G. Voglis, M. Vlachos, C. Fotakis, and N. Tavernarakis, “In vivo imaging of neurodegeneration in Caenorhabditis elegans by third harmonic generation microscopy,” J. Microsc. 232(2), 270–275 (2008). [PubMed]
  15. S. H. Chung and E. Mazur, “Femtosecond laser ablation of neurons in C. elegans for behavioral studies,” Appl. Phys., A Mater. Sci. Process. 96(2), 335–341 (2009). [CrossRef]
  16. M. F. Yanik, H. Cinar, H. N. Cinar, A. Chisholm, Y. Jin, and A. Ben-Yakar, “Nerve regeneration in Caenorhabditis elegans after femtosecond laser axotomy,” IEEE J. Quantum Electron. 12, 1283–1291 (2006). [CrossRef]
  17. F. Zeng, C. B. Rohde, and M. F. Yanik, “Sub-cellular precision on-chip small-animal immobilization, multi-photon imaging and femtosecond-laser manipulation,” Lab Chip 8(5), 653–656 (2008). [CrossRef] [PubMed]
  18. M. Mathew, S. I. C. O. Santos, D. Zalvidea, and P. Loza-Alvarez, “Multimodal optical workstation for simultaneous linear, nonlinear microscopy and nanomanipulation: upgrading a commercial confocal inverted microscope,” Rev. Sci. Instrum. 80(7), 073701 (2009). [CrossRef] [PubMed]
  19. http://www.nikoninstruments.com/Products/Microscope-Systems/Inverted- Microscopes/Biological/Eclipse-Ti .
  20. S. Brenner, “The genetics of Caenorhabditis elegans,” Genetics 77(1), 71–94 (1974). [PubMed]
  21. F. Bourgeois and A. Ben-Yakar, “Femtosecond laser nanoaxotomy properties and their effect on axonal recovery in C. elegans,” Opt. Express 16(8), 5963–15963 (2008), http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-8-5963 . [CrossRef] [PubMed]
  22. A. Vogel, J. Noack, G. Hüttmann, and G. Paltauf, “Mechanisms of femtosecond laser nanosurgery of cells and tissues,” Appl. Phys. B 81(8), 1015–1047 (2005). [CrossRef]
  23. N. I. Smith, Y. Kumamoto, S. Iwanaga, J. Ando, K. Fujita, and S. Kawata, “A femtosecond laser pacemaker for heart muscle cells,” Opt. Express 16(12), 8604–8616 (2008), http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-12-8604 . [CrossRef] [PubMed]
  24. M. C. Hogan, C. M. Stary, R. S. Balaban, and C. A. Combs, “NAD(P)H fluorescence imaging of mitochondrial metabolism in contracting Xenopus skeletal muscle fibers: effect of oxygen availability,” Appl. Physiol 98(4), 1420–1426 (2005). [CrossRef]

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