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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 2 — Jan. 21, 2010

Real time imaging of femtosecond laser induced nano-neurosurgery dynamics in C. elegans

Susana I. C. O. Santos, Manoj Mathew, and Pablo Loza-Alvarez  »View Author Affiliations


Optics Express, Vol. 18, Issue 1, pp. 364-377 (2010)
http://dx.doi.org/10.1364/OE.18.000364


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Abstract

In this study we present for the first time the use of confocal microscopy and laser scanning brightfield microscopy (LSBF) for real time imaging of femtosecond laser nanosurgery and its dynamics in C. elegans. A single multimodal optical workstation that provides the ability to perform femtosecond laser nanosurgery and simultaneous confocal and LSBF imaging was used for the purpose. With this tool several dynamic phenomena concomitant with laser nanosurgery in C. elegans were observed and imaged. Some of these dynamic phenomena, like muscular contraction and single muscle cell stimulation, have been imaged for the first time during nano-neurosurgery of C. elegans.

© 2009 OSA

OCIS Codes
(170.1020) Medical optics and biotechnology : Ablation of tissue
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: November 3, 2009
Revised Manuscript: December 18, 2009
Manuscript Accepted: December 18, 2009
Published: December 24, 2009

Virtual Issues
Vol. 5, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Susana I. C. O. Santos, Manoj Mathew, and Pablo Loza-Alvarez, "Real time imaging of femtosecond laser induced nano-neurosurgery dynamics in C. elegans," Opt. Express 18, 364-377 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-18-1-364


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