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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 10 — Jul. 19, 2010

Automated suppression of sample-related artifacts in Fluorescence Correlation Spectroscopy

Jonas Ries, Mathias Bayer, Gábor Csúcs, Ronald Dirkx, Michele Solimena, Helge Ewers, and Petra Schwille  »View Author Affiliations


Optics Express, Vol. 18, Issue 11, pp. 11073-11082 (2010)
http://dx.doi.org/10.1364/OE.18.011073


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Abstract

Fluorescence Correlation Spectroscopy (FCS) in cells often suffers from artifacts caused by bright aggregates or vesicles, depletion of fluorophores or bleaching of a fluorescent background. The common practice of manually discarding distorted curves is time consuming and subjective. Here we demonstrate the feasibility of automated FCS data analysis with efficient rejection of corrupted parts of the signal. As test systems we use a solution of fluorescent molecules, contaminated with bright fluorescent beads, as well as cells expressing a fluorescent protein (ICA512-EGFP), which partitions into bright secretory granules. This approach improves the accuracy of FCS measurements in biological samples, extends its applicability to especially challenging systems and greatly simplifies and accelerates the data analysis.

© 2010 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1420) Medical optics and biotechnology : Biology
(170.1530) Medical optics and biotechnology : Cell analysis
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(180.0180) Microscopy : Microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: March 30, 2010
Revised Manuscript: April 19, 2010
Manuscript Accepted: April 21, 2010
Published: May 11, 2010

Virtual Issues
Vol. 5, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Jonas Ries, Mathias Bayer, Gábor Csúcs, Ronald Dirkx, Michele Solimena, Helge Ewers, and Petra Schwille, "Automated suppression of sample-related artifacts in Fluorescence Correlation Spectroscopy," Opt. Express 18, 11073-11082 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-18-11-11073


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