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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 5, Iss. 14 — Nov. 16, 2010

An insight into statistical refractive index properties of cell internal structure via low-coherence statistical amplitude microscopy

Pin Wang, Rajan K. Bista, Wei Qiu, Walid E. Khalbuss, Lin Zhang, Randall E. Brand, and Yang Liu  »View Author Affiliations

Optics Express, Vol. 18, Issue 21, pp. 21950-21958 (2010)

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Refractive index properties, especially at the nanoscale, have shown great potential in cancer diagnosis and screening. Due to the intrinsic complexity and weak refractive index fluctuation, the reconstruction of internal structures of a biological cell has been challenging. In this paper, we propose a simple and practical approach to derive the statistical properties of internal refractive index fluctuations within a biological cell with a new optical microscopy method – Low-coherence Statistical Amplitude Microscopy (SAM). We validated the capability of SAM to characterize the statistical properties of cell internal structures, which is described by numerical models of one-dimensional Gaussian random field. We demonstrated the potential of SAM in cancer detection with an animal model of intestinal carcinogenesis – multiple intestinal neoplasia mouse model. We showed that SAM-derived statistical properties of cell nuclear structures could detect the subtle changes that are otherwise undetectable by conventional cytopathology.

© 2010 OSA

OCIS Codes
(110.0180) Imaging systems : Microscopy
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(290.1350) Scattering : Backscattering

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: August 30, 2010
Revised Manuscript: September 24, 2010
Manuscript Accepted: September 26, 2010
Published: September 30, 2010

Virtual Issues
Vol. 5, Iss. 14 Virtual Journal for Biomedical Optics

Pin Wang, Rajan K. Bista, Wei Qiu, Walid E. Khalbuss, Lin Zhang, Randall E. Brand, and Yang Liu, "An insight into statistical refractive index properties of cell internal structure via low-coherence statistical amplitude microscopy," Opt. Express 18, 21950-21958 (2010)

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