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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 5, Iss. 14 — Nov. 16, 2010

Coherence-controlled holographic microscope

Pavel Kolman and Radim Chmelík  »View Author Affiliations

Optics Express, Vol. 18, Issue 21, pp. 21990-22003 (2010)

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Transmitted-light coherence-controlled holographic microscope (CCHM) based on an off-axis achromatic interferometer allows us to use light sources of arbitrary degree of temporal and spatial coherence. Besides the conventional DHM modes such as quantitative phase contrast imaging and numerical 3D holographic reconstruction it provides high quality (speckle-free) imaging, improved lateral resolution and optical sectioning by coherence gating. Optical setup parameters and their limits for a technical realization are derived and described in detail. To demonstrate the optical sectioning property of the microscope a model sample uncovered and then covered with a diffuser was observed using a low-coherence light source.

© 2010 OSA

OCIS Codes
(090.0090) Holography : Holography
(110.4980) Imaging systems : Partial coherence in imaging
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(170.1790) Medical optics and biotechnology : Confocal microscopy
(180.3170) Microscopy : Interference microscopy
(110.0113) Imaging systems : Imaging through turbid media

ToC Category:

Original Manuscript: July 21, 2010
Revised Manuscript: September 13, 2010
Manuscript Accepted: September 16, 2010
Published: October 1, 2010

Virtual Issues
Vol. 5, Iss. 14 Virtual Journal for Biomedical Optics

Pavel Kolman and Radim Chmelík, "Coherence-controlled holographic microscope," Opt. Express 18, 21990-22003 (2010)

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