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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 5, Iss. 14 — Nov. 16, 2010

Twin-photon confocal microscopy

D. S. Simon and A. V. Sergienko  »View Author Affiliations

Optics Express, Vol. 18, Issue 21, pp. 22147-22157 (2010)

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A recently introduced two-channel confocal microscope with correlated detection promises up to 50% improvement in transverse spatial resolution [Simon, Sergienko, Optics Express 18, 9765 (2010)] via the use of photon correlations. Here we achieve similar results in a different manner, introducing a triple-confocal correlated microscope which exploits the correlations present in optical parametric amplifiers. It is based on tight focusing of pump radiation onto a thin sample positioned in front of a nonlinear crystal, followed by coincidence detection of signal and idler photons, each focused onto a pinhole. This approach offers further resolution enhancement in confocal microscopy.

© 2010 Optical Society of America

OCIS Codes
(180.1790) Microscopy : Confocal microscopy
(180.5810) Microscopy : Scanning microscopy
(190.4970) Nonlinear optics : Parametric oscillators and amplifiers

ToC Category:

Original Manuscript: August 30, 2010
Manuscript Accepted: September 19, 2010
Published: October 5, 2010

Virtual Issues
Vol. 5, Iss. 14 Virtual Journal for Biomedical Optics

D. S. Simon and A. V. Sergienko, "Twin-photon confocal microscopy," Opt. Express 18, 22147-22157 (2010)

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  1. [] D. S. Simon and A. V. Sergienko, ”The Correlation Confocal Microscope,” Opt. Express 18, 9765-9779 (2010). [CrossRef] [PubMed]
  2. [] W. Denk, J. Strickler, and W. W. Webb, ”Two-photon Laser Scanning Fluorescence Microscopy,” Science 248, 73-76 (1990). [CrossRef] [PubMed]
  3. [] W. Denk and K. Svoboda, Photon Upmanship: Why Multiphoton Imaging is More than a Gimmick, Neuron 18, 351-357 (1997). [CrossRef] [PubMed]
  4. [] T.B. Pittman, D.V. Strekalov, D.N. Klyshko, M.H. Rubin, A.V. Sergienko, Y.H. Shih, ”Two-photon geometric optics,” Phys. Rev. A 53, 2804-2815 (1996). [CrossRef] [PubMed]
  5. [] W. Lukosz, ”Optical systems with resolving powers exceeding the classical limit”, J. Opt. Soc. Am. 561463-1472 (1966). [CrossRef]
  6. Q1[] R. Heintzmann, M.G.L. Gustafsson, ”Subdiffraction resolution in continuous samples”, Nat. Photonics 3, 362-364 (2009). [CrossRef]
  7. [] P. Török, T. Wilson, ”Rigorous theory for axial resolution in confocal microscopes”, Opt. Commun. 137, 127-135 (1997). [CrossRef]
  8. [] T. Wilson, R. Ju?skaitis, and P. Higdon, ”The imaging of dielectric point scatterers in conventional and confocal microscopes”, Opt. Commun. 141, 298-313 (1997). [CrossRef]
  9. [] P. Török, P. D. Higdon, T. Wilson, ”On the general properties of polarized light conventional and confocal microscopes”, Opt. Commun. 148, 300-315 (1998). [CrossRef]
  10. [] P. Török, P. D. Higdon, T. Wilson, ”Theory for confocal and conventional microscopes imaging small dielectric scatterers”, J. Mod. Opt. 45, 1681-1698 (1998). [CrossRef]
  11. [] C. J. R. Sheppard and J. Felix Aguilar, ”Electromagnetic imaging in the confocal microscope”, Opt. Commun. 180, 1-8 (2000). [CrossRef]

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