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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 5, Iss. 14 — Nov. 16, 2010

Removal of subsurface fluorescence in cryo-imaging using deconvolution

Ganapathy Krishnamurthi, Charlie Y. Wang, Grant Steyer, and David L. Wilson  »View Author Affiliations


Optics Express, Vol. 18, Issue 21, pp. 22324-22338 (2010)
http://dx.doi.org/10.1364/OE.18.022324


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Abstract

We compared image restoration methods [Richardson-Lucy (RL), Wiener, and Next-image] with measured “scatter” point-spread-functions, for removing subsurface fluorescence from section-and-image cryo-image volumes. All methods removed haze, delineated single cells from clusters, and improved visualization, but RL best represented structures. Contrast-to-noise and contrast-to-background improvement from RL and Wiener were comparable and 35% better than Next-image. Concerning detection of labeled cells, ROC analyses showed RL ≈Wiener > Next-image >> no processing. Next-image was faster than other methods and less prone to image processing artifacts. RL is recommended for the best restoration of the shape and size of fluorescent structures.

© 2010 OSA

OCIS Codes
(100.0100) Image processing : Image processing
(100.1830) Image processing : Deconvolution
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Image Processing

History
Original Manuscript: August 6, 2010
Revised Manuscript: September 12, 2010
Manuscript Accepted: September 20, 2010
Published: October 7, 2010

Virtual Issues
Vol. 5, Iss. 14 Virtual Journal for Biomedical Optics

Citation
Ganapathy Krishnamurthi, Charlie Y. Wang, Grant Steyer, and David L. Wilson, "Removal of subsurface fluorescence in cryo-imaging using deconvolution," Opt. Express 18, 22324-22338 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-18-21-22324


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