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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 6 — Jul. 7, 2011

Single-cell isolation using a DVD optical pickup

A. Kasukurti, M. Potcoava, S.A. Desai, C. Eggleton, and D. W. M. Marr  »View Author Affiliations

Optics Express, Vol. 19, Issue 11, pp. 10377-10386 (2011)

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A low-cost single-cell isolation system incorporating a digital versatile disc burner (DVD RW) optical pickup has been developed. We show that these readily available modules have the required laser power and focusing optics to provide a steady Gaussian beam capable of optically trapping micron-sized colloids and red blood cells. Utility of the pickup is demonstrated through the non-destructive isolation of such particles in a laminar-flow based microfluidic device that captures and translates single microscale objects across streamlines into designated channel exits. In this, the integrated objective lens focusing coils are used to steer the optical trap across the channel, resulting in the isolation of colloids and red blood cells using a very inexpensive off-the-shelf optical component.

© 2011 OSA

OCIS Codes
(140.2020) Lasers and laser optics : Diode lasers
(140.5960) Lasers and laser optics : Semiconductor lasers
(140.7010) Lasers and laser optics : Laser trapping
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.0180) Medical optics and biotechnology : Microscopy
(170.1530) Medical optics and biotechnology : Cell analysis
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: February 23, 2011
Revised Manuscript: April 9, 2011
Manuscript Accepted: April 11, 2011
Published: May 11, 2011

Virtual Issues
Vol. 6, Iss. 6 Virtual Journal for Biomedical Optics

A. Kasukurti, M. Potcoava, S.A. Desai, C. Eggleton, and D. W. M. Marr, "Single-cell isolation using a DVD optical pickup," Opt. Express 19, 10377-10386 (2011)

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  1. N. Uchida, D. W. Buck, D. He, M. J. Reitsma, M. Masek, T. V. Phan, A. S. Tsukamoto, F. H. Gage, and I. L. Weissman, “Direct isolation of human central nervous system stem cells,” Proc. Natl. Acad. Sci. U.S.A. 97, 14720–14725 (2000). [CrossRef] [PubMed]
  2. T. Kekarainen, S. Mannelin, J. Laine, and T. Jaatinen, “Optimization of immunomagnetic separation for cord blood-derived hematopoietic stem cells,” BMC Cell Biol. 7(1), 1–10 (2006). [CrossRef]
  3. S. Sieben, C. Bergemann, A. Lubbe, B. Brockmann, and D. Rescheleit, “Comparison of different particles and methods for magnetic isolation of circulating tumor cells,” J. Magn. Magn. Mater. 225(1-2), 175–179 (2001). [CrossRef]
  4. K. Dotan, B. Feldman, B. Goldman, Y. Peri, and L. Peleg, “The single cell as a tool for genetic testing: credibility, precision, implication,” J. Assist. Reprod. Genet. 27(6), 335–341 (2010). [CrossRef] [PubMed]
  5. S. C. Bhakdi, A. Ottinger, S. Somsri, P. Sratongno, P. Pannadaporn, P. Chimma, P. Malasit, K. Pattanapanyasat, and H. P. H. Neumann, “Optimized high gradient magnetic separation for isolation of Plasmodium-infected red blood cells,” Malar. J. 9(1), 30–38 (2010). [CrossRef]
  6. N. Beyor, T. S. Seo, P. Liu, and R. A. Mathies, “Immunomagnetic bead-based cell concentration microdevice for dilute pathogen detection,” Biomed. Microdevices 10(6), 909–917 (2008). [CrossRef] [PubMed]
  7. F. L. Battye, A. Light, and D. M. Tarlinton, “Single cell sorting and cloning,” J. Immunol. Methods 243(1-2), 25–32 (2000). [CrossRef] [PubMed]
  8. C. E. M. Sharrock, E. Kaminski, and S. Man, “Limiting dilution analysis of human T cells: a useful clinical tool,” Immunol. Today 11(8), 281–286 (1990). [CrossRef] [PubMed]
  9. R. S. W. Thomas, P. D. Mitchell, R. O. C. Oreffo, and H. Morgan, “Trapping single human osteoblast-like cells from a heterogeneous population using a dielectrophoretic microfluidic device,” Biomicrofluidics 4(2), 022806 (2010). [CrossRef]
  10. F. Yang, X. M. Yang, H. Jiang, P. Bulkhaults, P. Wood, W. Hrushesky, and G. R. Wang, “Dielectrophoretic separation of colorectal cancer cells,” Biomicrofluidics 4(1), 013204 (2010). [CrossRef]
  11. A. Rosenthal and J. Voldman, “Dielectrophoretic traps for single-particle patterning,” Biophys. J. 88(3), 2193–2205 (2005). [CrossRef]
  12. J. Oakey, J. Allely, and D. W. M. Marr, “Laminar-flow-based separations at the microscale,” Biotechnol. Prog. 18(6), 1439–1442 (2002). [CrossRef] [PubMed]
  13. S. H. Cho, C. H. Chen, F. S. Tsai, J. M. Godin, and Y. H. Lo, “Human mammalian cell sorting using a highly integrated micro-fabricated fluorescence-activated cell sorter (microFACS),” Lab Chip 10(12), 1567–1573 (2010). [CrossRef] [PubMed]
  14. S. L. Windler-Hart, K. Y. Chen, and A. Chenn, “A cell behavior screen: identification, sorting, and enrichment of cells based on motility,” BMC Cell Biol. 6(1), 14–23 (2005). [CrossRef] [PubMed]
  15. R. W. Applegate, D. Schafer, W. Amir, J. Squier, T. Vestad, J. Oakey, and D. W. M. Marr, “Optically-integrated microfluidic systems for cellular characterization and manipulation,” J. Opt. A, Pure Appl. Opt. 9(8), S122–S128 (2007). [CrossRef]
  16. R. W. Applegate, J. Squier, T. Vestad, J. Oakey, and D. W. M. Marr, “Optical trapping, manipulation, and sorting of cells and colloids in microfluidic systems with diode laser bars,” Opt. Express 12(19), 4390–4398 (2004). [CrossRef] [PubMed]
  17. R. W. Applegate, J. Squier, T. Vestad, J. Oakey, and D. W. M. Marr, “Fiber-focused diode-bar optical trapping for microfluidic flow manipulation,” Appl. Phys. Lett. 92(1), 013904 (2008). [CrossRef]
  18. R. W. Applegate, J. Squier, T. Vestad, J. Oakey, D. W. M. Marr, P. Bado, M. A. Dugan, and A. A. Said, “Microfluidic sorting system based on optical waveguide integration and diode laser bar trapping,” Lab Chip 6(3), 422–426 (2006). [CrossRef] [PubMed]
  19. K. Immink, “The compact disk story,” J. Audio Eng. Soc. 46, 458–465 (1998).
  20. F. Quercioli, B. Tiribilli, and A. Bartoli, “Interferometry with optical pickups,” Opt. Lett. 24(10), 670–672 (1999). [CrossRef]
  21. K. H. Kim, S. Y. Lee, S. Kim, S. H. Lee, and S. G. Jeong, “A new DNA chip detection mechanism using optical pick-up actuators,” Microsyst. Technol. 13(8-10), 1359–1369 (2007). [CrossRef]
  22. K. C. Fan, C. L. Chu, J. L. Liao, and J. I. Mou, “Development of a high-precision straightness measuring system with DVD pick-up head,” Meas. Sci. Technol. 14(1), 47–54 (2003). [CrossRef]
  23. C. L. Chu and C. H. Lin, “Development of an optical accelerometer with a DVD pick-up head,” Meas. Sci. Technol. 16(12), 2498–2502 (2005). [CrossRef]
  24. C. L. Chu, C. H. Lin, and K. C. Fan, “Two-dimensional optical accelerometer based on commercial DVD pick-up head,” Meas. Sci. Technol. 18(1), 265–274 (2007). [CrossRef]
  25. E. T. Hwu, S. K. Hung, C. W. Yang, K. Y. Huang, and I. S. Hwang, “Real-time detection of linear and angular displacements with a modified DVD optical head,” Nanotechnology 19(11), 115501 (2008). [CrossRef] [PubMed]
  26. R. A. Potyrailo, W. G. Morris, A. M. Leach, L. Hassib, K. Krishnan, C. Surman, R. Wroczynski, S. Boyette, C. Xiao, P. Shrikhande, A. Agree, and T. Cecconie, “Theory and practice of ubiquitous quantitative chemical analysis using conventional computer optical disk drives,” Appl. Opt. 46(28), 7007–7017 (2007). [CrossRef] [PubMed]
  27. S. A. Lange, G. Roth, S. Wittemann, T. Lacoste, A. Vetter, J. Grässle, S. Kopta, M. Kolleck, B. Breitinger, M. Wick, J. K. H. Hörber, S. Dübel, and A. Bernard, “Measuring biomolecular binding events with a compact disc player device,” Angew. Chem. Int. Ed. Engl. 45(2), 270–273 (2006). [CrossRef]
  28. K. H. Kim, S. Y. Lee, S. Kim, and S. G. Jeong, “DNA microarray scanner with a DVD pick-up head,” Curr. Appl. Phys. 8(6), 687–691 (2008). [CrossRef]
  29. J. Benschop and G. van Rosmalen, “Confocal compact scanning optical microscope based on compact disc technology,” Appl. Opt. 30(10), 1179–1184 (1991). [CrossRef] [PubMed]
  30. S. Kostner and M. Vellekoop, “Cell analysis in a microfluidic cytorneter applying a DVD pickup head,” Sens. Actuators B Chem. 132(2), 512–517 (2008). [CrossRef]
  31. D. C. Duffy, J. C. McDonald, O. J. A. Schueller, and G. M. Whitesides, “Rapid prototyping of microfluidic systems in poly(dimethylsiloxane),” Anal. Chem. 70(23), 4974–4984 (1998). [CrossRef] [PubMed]
  32. J. Happel and H. Brenner, Low Reynolds Number Hydrodynamics (Prentice-Hall, Inc., 1965).
  33. M. Miwa, S. Juodkazis, and H. Misawa, “Drag of a laser trapped fine particle in a microregion,” Jpn. J. Appl. Phys. 39(Part 1, No. 4A), 1930–1933 (2000). [CrossRef]

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