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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 11 — Oct. 31, 2012

Adaptive optics enables 3D STED microscopy in aberrating specimens

Travis J. Gould, Daniel Burke, Joerg Bewersdorf, and Martin J. Booth  »View Author Affiliations


Optics Express, Vol. 20, Issue 19, pp. 20998-21009 (2012)
http://dx.doi.org/10.1364/OE.20.020998


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Abstract

Stimulated emission depletion (STED) microscopy allows fluorescence far-field imaging with diffraction-unlimited resolution. Unfortunately, extending this technique to three-dimensional (3D) imaging of thick specimens has been inhibited by sample-induced aberrations. Here we present the first implementation of adaptive optics in STED microscopy to allow 3D super-resolution imaging in strongly aberrated imaging conditions, such as those introduced by thick biological tissue.

© 2012 OSA

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(350.5730) Other areas of optics : Resolution
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Microscopy

History
Original Manuscript: June 29, 2012
Revised Manuscript: July 31, 2012
Manuscript Accepted: August 2, 2012
Published: August 29, 2012

Virtual Issues
Vol. 7, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Travis J. Gould, Daniel Burke, Joerg Bewersdorf, and Martin J. Booth, "Adaptive optics enables 3D STED microscopy in aberrating specimens," Opt. Express 20, 20998-21009 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-20-19-20998


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