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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 3 — Feb. 29, 2012

Accuracy of correction in modal sensorless adaptive optics

Aurélie Facomprez, Emmanuel Beaurepaire, and Delphine Débarre  »View Author Affiliations

Optics Express, Vol. 20, Issue 3, pp. 2598-2612 (2012)

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We investigate theoretically and experimentally the parameters governing the accuracy of correction in modal sensorless adaptive optics for microscopy. On the example of two-photon fluorescence imaging, we show that using a suitable number of measurements, precise correction can be obtained for up to 2 radians rms aberrations without optimising the aberration modes used for correction. We also investigate the number of photons required for accurate correction when signal acquisition is shot-noise limited. We show that only 104 to 105 photons are required for complete correction so that the correction process can be implemented with limited extra-illumination and associated photoperturbation. Finally, we provide guidelines for implementing an optimal correction algorithm depending on the experimental conditions.

© 2012 OSA

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.6900) Microscopy : Three-dimensional microscopy
(180.4315) Microscopy : Nonlinear microscopy
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Adaptive Optics

Original Manuscript: November 23, 2011
Revised Manuscript: January 2, 2012
Manuscript Accepted: January 10, 2012
Published: January 20, 2012

Virtual Issues
Vol. 7, Iss. 3 Virtual Journal for Biomedical Optics

Aurélie Facomprez, Emmanuel Beaurepaire, and Delphine Débarre, "Accuracy of correction in modal sensorless adaptive optics," Opt. Express 20, 2598-2612 (2012)

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