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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 3 — Feb. 29, 2012

Time-resolved protein nanocrystallography using an X-ray free-electron laser

Andrew Aquila, Mark S. Hunter, R. Bruce Doak, Richard A. Kirian, Petra Fromme, Thomas A. White, Jakob Andreasson, David Arnlund, Saša Bajt, Thomas R. M. Barends, Miriam Barthelmess, Michael J. Bogan, Christoph Bostedt, Hervé Bottin, John D. Bozek, Carl Caleman, Nicola Coppola, Jan Davidsson, Daniel P. DePonte, Veit Elser, Sascha W. Epp, Benjamin Erk, Holger Fleckenstein, Lutz Foucar, Matthias Frank, Raimund Fromme, Heinz Graafsma, Ingo Grotjohann, Lars Gumprecht, Janos Hajdu, Christina Y. Hampton, Andreas Hartmann, Robert Hartmann, Stefan Hau-Riege, Günter Hauser, Helmut Hirsemann, Peter Holl, James M. Holton, André Hömke, Linda Johansson, Nils Kimmel, Stephan Kassemeyer, Faton Krasniqi, Kai-Uwe Kühnel, Mengning Liang, Lukas Lomb, Erik Malmerberg, Stefano Marchesini, Andrew V. Martin, Filipe R.N.C. Maia, Marc Messerschmidt, Karol Nass, Christian Reich, Richard Neutze, Daniel Rolles, Benedikt Rudek, Artem Rudenko, Ilme Schlichting, Carlo Schmidt, Kevin E. Schmidt, Joachim Schulz, M. Marvin Seibert, Robert L. Shoeman, Raymond Sierra, Heike Soltau, Dmitri Starodub, Francesco Stellato, Stephan Stern, Lothar Strüder, Nicusor Timneanu, Joachim Ullrich, Xiaoyu Wang, Garth J. Williams, Georg Weidenspointner, Uwe Weierstall, Cornelia Wunderer, Anton Barty, John C. H. Spence, and Henry N. Chapman  »View Author Affiliations


Optics Express, Vol. 20, Issue 3, pp. 2706-2716 (2012)
http://dx.doi.org/10.1364/OE.20.002706


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Abstract

We demonstrate the use of an X-ray free electron laser synchronized with an optical pump laser to obtain X-ray diffraction snapshots from the photoactivated states of large membrane protein complexes in the form of nanocrystals flowing in a liquid jet. Light-induced changes of Photosystem I-Ferredoxin co-crystals were observed at time delays of 5 to 10 µs after excitation. The result correlates with the microsecond kinetics of electron transfer from Photosystem I to ferredoxin. The undocking process that follows the electron transfer leads to large rearrangements in the crystals that will terminally lead to the disintegration of the crystals. We describe the experimental setup and obtain the first time-resolved femtosecond serial X-ray crystallography results from an irreversible photo-chemical reaction at the Linac Coherent Light Source. This technique opens the door to time-resolved structural studies of reaction dynamics in biological systems.

© 2012 OSA

OCIS Codes
(140.3450) Lasers and laser optics : Laser-induced chemistry
(140.7090) Lasers and laser optics : Ultrafast lasers
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.7160) Medical optics and biotechnology : Ultrafast technology
(170.7440) Medical optics and biotechnology : X-ray imaging

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: November 11, 2011
Revised Manuscript: December 16, 2011
Manuscript Accepted: December 18, 2011
Published: January 23, 2012

Virtual Issues
Vol. 7, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Andrew Aquila, Mark S. Hunter, R. Bruce Doak, Richard A. Kirian, Petra Fromme, Thomas A. White, Jakob Andreasson, David Arnlund, Saša Bajt, Thomas R. M. Barends, Miriam Barthelmess, Michael J. Bogan, Christoph Bostedt, Hervé Bottin, John D. Bozek, Carl Caleman, Nicola Coppola, Jan Davidsson, Daniel P. DePonte, Veit Elser, Sascha W. Epp, Benjamin Erk, Holger Fleckenstein, Lutz Foucar, Matthias Frank, Raimund Fromme, Heinz Graafsma, Ingo Grotjohann, Lars Gumprecht, Janos Hajdu, Christina Y. Hampton, Andreas Hartmann, Robert Hartmann, Stefan Hau-Riege, Günter Hauser, Helmut Hirsemann, Peter Holl, James M. Holton, André Hömke, Linda Johansson, Nils Kimmel, Stephan Kassemeyer, Faton Krasniqi, Kai-Uwe Kühnel, Mengning Liang, Lukas Lomb, Erik Malmerberg, Stefano Marchesini, Andrew V. Martin, Filipe R.N.C. Maia, Marc Messerschmidt, Karol Nass, Christian Reich, Richard Neutze, Daniel Rolles, Benedikt Rudek, Artem Rudenko, Ilme Schlichting, Carlo Schmidt, Kevin E. Schmidt, Joachim Schulz, M. Marvin Seibert, Robert L. Shoeman, Raymond Sierra, Heike Soltau, Dmitri Starodub, Francesco Stellato, Stephan Stern, Lothar Strüder, Nicusor Timneanu, Joachim Ullrich, Xiaoyu Wang, Garth J. Williams, Georg Weidenspointner, Uwe Weierstall, Cornelia Wunderer, Anton Barty, John C. H. Spence, and Henry N. Chapman, "Time-resolved protein nanocrystallography using an X-ray free-electron laser," Opt. Express 20, 2706-2716 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-20-3-2706


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