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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 7 — Jul. 17, 2006

Combined depth- and time-resolved autofluorescence spectroscopy of epithelial tissue

Yicong Wu and Jianan Y. Qu  »View Author Affiliations


Optics Letters, Vol. 31, Issue 12, pp. 1833-1835 (2006)
http://dx.doi.org/10.1364/OL.31.001833


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Abstract

A time-resolved confocal fluorescence spectroscopy system is built to measure the fine structure and localized biochemistry of epithelial tissue. It is found that the autofluorescence excited at 405 nm is sensitive to the cellular metabolism and can be used to sense the metabolic status of epithelial tissue. The decay of autofluorescence excited at 405 nm can be accurately fitted with a dual-exponential function consisting of short lifetime ( 0.4 0.6 ns ) and long lifetime ( 3 4 ns ) components. The ratio of the amplitudes of the two components provides information on the fine structure of epithelial tissue. We demonstrate that the combined depth- and time-resolved measurements with single excitation can potentially provide accurate information for the diagnosis of tissue pathology.

© 2006 Optical Society of America

OCIS Codes
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(300.6500) Spectroscopy : Spectroscopy, time-resolved

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: March 28, 2006
Manuscript Accepted: April 6, 2006

Virtual Issues
Vol. 1, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Yicong Wu and Jianan Y. Qu, "Combined depth- and time-resolved autofluorescence spectroscopy of epithelial tissue," Opt. Lett. 31, 1833-1835 (2006)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-31-12-1833


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References

  1. N. Ramanujam, in Encyclopedia of Analytical Chemistry, R.A.Meyers, ed. (Wiley, 2000), pp. 20-56.
  2. Y. Wu, P. Xi, J. Y. Qu, T. Cheung, and M. Yu, Opt. Express 12, 3218 (2004). [CrossRef] [PubMed]
  3. Y. Wu, P. Xi, J. Y. Qu, T. Cheung, and M. Yu, Opt. Express 13, 382 (2005). [CrossRef] [PubMed]
  4. J. R. Lakowicz, Principles of Fluorescence Spectroscopy (Kluwer Academic/Plenum, 1999).
  5. K. Vishwanath and M.-A. Mycek, Opt. Lett. 29, 1512 (2004). [CrossRef] [PubMed]
  6. Y. Wu and J. Y. Qu, Opt. Lett. 30, 3045 (2005). [CrossRef] [PubMed]
  7. N. D. Kirkpatrick, C. Zou, M. A. Brewer, W. R. Brands, R. A. Drezek, and U. Utzinger, Photochem. Photobiol. 81, 125 (2005). [CrossRef]
  8. K. Sokolo, J. Galvan, A. Myakov, A. Lacy, R. Lotan, and R. Richard-Kortum, J. Biomed. Opt. 7, 148 (2002). [CrossRef]
  9. S. Huang, A. A. Heikal, and W. W. Webb, Biophys. J. 82, 2811 (2002). [CrossRef] [PubMed]
  10. K. Konig and I. Riemann, J. Biomed. Opt. 8, 432 (2003). [CrossRef] [PubMed]
  11. Q. Fang, T. Papaioannou, J. A. Jo, R. Vaitha, K. Shastry, and L. Marcu, Rev. Sci. Instrum. 75, 151 (2004). [CrossRef]
  12. H. Schneckenburger and K. Konig, Opt. Eng. 31, 1447 (1992). [CrossRef]

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