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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 9 — Sep. 26, 2007

Increasing the imaging depth of coherent anti-Stokes Raman scattering microscopy with a miniature microscope objective

Haifeng Wang, Terry B. Huff, Yan Fu, Kevin Y. Jia, and Ji-Xin Cheng  »View Author Affiliations


Optics Letters, Vol. 32, Issue 15, pp. 2212-2214 (2007)
http://dx.doi.org/10.1364/OL.32.002212


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Abstract

A miniature objective lens with a tip diameter of 1.3 mm was used for extending the penetration depth of coherent anti-Stokes Raman scattering (CARS) microscopy. Its axial and lateral focal widths were determined to be 11.4 and 0.86 μ m , respectively, by two-photon excitation fluorescence imaging of 200 nm beads at a 735 nm excitation wavelength. By inserting the lens tip into a soft gel sample, CARS images of 2 μ m polystyrene beads 5 mm deep from the surface were acquired. The miniature objective was applied to CARS imaging of rat spinal cord white matter with a minimal requirement for surgery.

© 2007 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(350.3950) Other areas of optics : Micro-optics

ToC Category:
Microscopy

History
Original Manuscript: March 29, 2007
Revised Manuscript: May 28, 2007
Manuscript Accepted: May 30, 2007
Published: July 24, 2007

Virtual Issues
Vol. 2, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Haifeng Wang, Terry B. Huff, Yan Fu, Kevin Y. Jia, and Ji-Xin Cheng, "Increasing the imaging depth of coherent anti-Stokes Raman scattering microscopy with a miniature microscope objective," Opt. Lett. 32, 2212-2214 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-32-15-2212


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References

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