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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 3 — Mar. 7, 2007

Phase retrieval in low-coherence interferometric microscopy

Audrey K. Ellerbee and Joseph A. Izatt  »View Author Affiliations

Optics Letters, Vol. 32, Issue 4, pp. 388-390 (2007)

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We present compensating methods that address inherent errors in quantitative phase reporting for low-coherence interferometric techniques. A brief theoretical treatment of the problem and experimental validation using spectral domain phase microscopy demonstrate mitigation of the degrading effects of phase leakage on accurate measurement of optical path length in the vicinity of closely spaced reflectors. This result has direct implications for phase-sensitive interferometry techniques, such as Doppler imaging, as well as amplitude-based quantitative reporting. Corrected phase retrieval is demonstrated for conversion of interferometric phase to optical path length in cell surface deflections of beating cardiomyocytes.

© 2007 Optical Society of America

OCIS Codes
(100.5070) Image processing : Phase retrieval
(110.0180) Imaging systems : Microscopy
(110.4280) Imaging systems : Noise in imaging systems
(110.4500) Imaging systems : Optical coherence tomography
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(180.3170) Microscopy : Interference microscopy

ToC Category:

Original Manuscript: August 29, 2006
Revised Manuscript: November 11, 2006
Manuscript Accepted: November 16, 2006
Published: January 26, 2007

Virtual Issues
Vol. 2, Iss. 3 Virtual Journal for Biomedical Optics

Audrey K. Ellerbee and Joseph A. Izatt, "Phase retrieval in low-coherence interferometric microscopy," Opt. Lett. 32, 388-390 (2007)

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