Miniaturization and defocus correction for objective-coupled planar illumination microscopy
Optics Letters, Vol. 33, Issue 20, pp. 2302-2304 (2008)
http://dx.doi.org/10.1364/OL.33.002302
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Abstract
Recently, a light sheet-based technique called objective-coupled planar illumination (OCPI) microscopy [ Holekamp et al., Neuron 57, 661 (2008) ] was shown to permit low-phototoxicity, high-speed, three-dimensional fluorescence imaging of extended tissue samples. Here, we introduce two major improvements in OCPI microscopy. First, we miniaturize the objective coupler by using a uniaxial gradient-index lens to produce the light sheet. Second, we demonstrate theoretically and experimentally that refractive index mismatch at the fluid/tissue interface causes a significant defocus aberration. By introducing the ability to tune the angle of the light sheet, we show that defocus correction in a miniaturized OCPI microscope leads to a significant improvement in image sharpness deeper into tissue.
© 2008 Optical Society of America
OCIS Codes
(110.2760) Imaging systems : Gradient-index lenses
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(170.2945) Medical optics and biotechnology : Illumination design
ToC Category:
Microscopy
History
Original Manuscript: July 17, 2008
Manuscript Accepted: August 14, 2008
Published: October 7, 2008
Virtual Issues
Vol. 3, Iss. 12 Virtual Journal for Biomedical Optics
Citation
Diwakar Turaga and Timothy E. Holy, "Miniaturization and defocus correction for objective-coupled planar illumination microscopy," Opt. Lett. 33, 2302-2304 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-33-20-2302
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