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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 2 — Feb. 10, 2009

Elliptically polarized coherent anti-Stokes Raman scattering microscopy

Fake Lu, Wei Zheng, and Zhiwei Huang  »View Author Affiliations


Optics Letters, Vol. 33, Issue 23, pp. 2842-2844 (2008)
http://dx.doi.org/10.1364/OL.33.002842


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Abstract

We report an approach to coherent anti-Stokes Raman scattering (CARS) microscopy that significantly suppresses the nonresonant background for high-contrast vibrational imaging. We employ an elliptically polarized pump field with an aspect ratio of 1 : 3 of the ellipse combined with a linearly polarized Stokes field for elliptically polarized CARS (EP-CARS) microscopy. The EP-CARS technique yields a 6–7-fold improvement in signal-to-background ratios compared with normal CARS, while providing an approximately 1.25-fold improvement in resonant CARS signal compared with normal polarization-sensitive CARS microscopy. We demonstrate this technique by imaging 1.5 μ m polystyrene beads in water and lipid droplets in unstained fibroblast cells.

© 2008 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.0180) Medical optics and biotechnology : Microscopy
(190.4380) Nonlinear optics : Nonlinear optics, four-wave mixing
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(100.3175) Image processing : Interferometric imaging
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Image Processing

History
Original Manuscript: August 4, 2008
Revised Manuscript: September 23, 2008
Manuscript Accepted: October 6, 2008
Published: November 25, 2008

Virtual Issues
Vol. 4, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Fake Lu, Wei Zheng, and Zhiwei Huang, "Elliptically polarized coherent anti-Stokes Raman scattering microscopy," Opt. Lett. 33, 2842-2844 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-33-23-2842

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