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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 2 — Feb. 10, 2009

Targeted photoporation and transfection in human HepG2 cells by a fiber femtosecond laser at 1554 nm

Hao He, Siu-Kai Kong, Rebecca Kit-Ying Lee, Yick-Keung Suen, and Kam Tai Chan  »View Author Affiliations


Optics Letters, Vol. 33, Issue 24, pp. 2961-2963 (2008)
http://dx.doi.org/10.1364/OL.33.002961


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Abstract

We report the transfection of human hepatocarcinoma (HepG2) cells by femtosecond (fs) laser pulses at 1554 nm . It was found that HepG2 cells could be perforated transiently to admit propidium iodide by that laser. This photoporation was safe, as the membrane resealed itself within a short time and no mitochondrial depolarization was detected. The cells were next photoporated in the presence of DNA plasmids for the expression of green fluorescence protein, and about 80% of the exposed cells showed green fluorescence 24 h later. Thus it could be concluded that it is safe and efficient to use fs laser at 1554 nm to transfect foreign molecules into cells under a standard microscope.

© 2008 Optical Society of America

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.7160) Medical optics and biotechnology : Ultrafast technology
(320.2250) Ultrafast optics : Femtosecond phenomena

ToC Category:
Ultrafast Optics

History
Original Manuscript: August 18, 2008
Revised Manuscript: October 22, 2008
Manuscript Accepted: October 24, 2008
Published: December 5, 2008

Virtual Issues
Vol. 4, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Hao He, Siu-Kai Kong, Rebecca Kit-Ying Lee, Yick-Keung Suen, and Kam Tai Chan, "Targeted photoporation and transfection in human HepG2 cells by a fiber femtosecond laser at 1554 nm," Opt. Lett. 33, 2961-2963 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-33-24-2961


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