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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 6 — Jun. 17, 2008

Determination of the three-dimensional orientation of single molecules

Matthew R. Foreman, Carlos Macías Romero, and Peter Török  »View Author Affiliations


Optics Letters, Vol. 33, Issue 9, pp. 1020-1022 (2008)
http://dx.doi.org/10.1364/OL.33.001020


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Abstract

A simple method for real-time determination of the full three-dimensional orientation of the emission dipole of single molecules is presented. Introduction of a π phase step in the back focal plane of the collector lens breaks the inherent symmetry, allowing the longitudinal dipole component to be measured. Experimental tolerances and the consequence of a nonzero bandwidth emission spectrum are also discussed. The scheme also allows for the longitudinal component of an electric field to be found experimentally.

© 2008 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(260.2510) Physical optics : Fluorescence
(300.2140) Spectroscopy : Emission

ToC Category:
Microscopy

History
Original Manuscript: February 1, 2008
Manuscript Accepted: March 31, 2008
Published: April 30, 2008

Virtual Issues
Vol. 3, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Matthew R. Foreman, Carlos Macías Romero, and Peter Török, "Determination of the three-dimensional orientation of single molecules," Opt. Lett. 33, 1020-1022 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-33-9-1020

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