OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 6 — Jun. 17, 2008

Imaging cortical electrical stimulation in vivo: fast intrinsic optical signal versus voltage-sensitive dyes

Vassiliy Tsytsarev, Kaushalya Premachandra, Daisuke Takeshita, and Sonya Bahar  »View Author Affiliations

Optics Letters, Vol. 33, Issue 9, pp. 1032-1034 (2008)

View Full Text Article

Enhanced HTML    Acrobat PDF (303 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We applied high-temporal-resolution optical imaging utilizing both the fast intrinsic optical signal (fIOS) and voltage-sensitive dyes (VSDs) to observe the spatiotemporal characteristics of rat somatosensory cortex during electrical stimulation. We find that changes in both the fIOS and VSD signals occur rapidly ( < 30 ms ) after the stimulus is applied, suggesting that both membrane depolarization and transmembrane ion movement occur shortly after the stimulus, preceding the more gradual physiological changes in oxygen consumption revealed by the slower component of the intrinsic optical signal. We find that the VSD signal spreads through a much larger area of cortex than the fIOS.

© 2008 Optical Society of America

OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.1420) Medical optics and biotechnology : Biology

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: January 15, 2008
Revised Manuscript: March 24, 2008
Manuscript Accepted: March 28, 2008
Published: April 30, 2008

Virtual Issues
Vol. 3, Iss. 6 Virtual Journal for Biomedical Optics

Vassiliy Tsytsarev, Kaushalya Premachandra, Daisuke Takeshita, and Sonya Bahar, "Imaging cortical electrical stimulation in vivo: fast intrinsic optical signal versus voltage-sensitive dyes," Opt. Lett. 33, 1032-1034 (2008)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. T. Bonhoeffer and A. Grinvald, in Brain Mapping: the Methods, A.W.Toga and J.C.Mazziota, eds. (Academic, 1996).
  2. D. Rector, R. Rogers, J. Schwaber, R. Harper, and J. George, Neuroimage 14, 977 (2001). [CrossRef] [PubMed]
  3. D. Shoham, D. E. Glaser, A. Arieli, T. Kenet, C. Wijnbergen, Y. Toledo, R. Hildesheim, and A. Grinvald, Neuron 24, 791 (1999). [CrossRef]
  4. A. M. Kuncel and W. M. Grill, Clin. Neurophysiol. 115, 2431 (2004). [CrossRef] [PubMed]
  5. S. D. Stoney, W. D. Thompson, and H. Asanuma, J. Neurophysiol. 31, 659 (1968). [PubMed]
  6. E. J. Tehovnik, A. S. Tolias, F. Sultan, W. M. Slocum, and N. K. Logothetis, J. Neurophysiol. 96, 512 (2006). [CrossRef] [PubMed]
  7. R. D. Andrew, C. R. Jarvis, and A. S. Obeidat, Methods 18, 185 (1998). [CrossRef]
  8. T. Basarsky, D. Feighan, and B. MacVicar, J. Neurosci. 19, 6439 (1999). [PubMed]
  9. E. Sykova, L. Vargova, S. Kubinova, P. Jendelova, and A. Chvátal, Neuroimage 18, 214 (2003). [CrossRef] [PubMed]
  10. B. A. MacVicar, D. Feighan, A. Brown, and B. Ransom, Glia 37, 114 (2002). [CrossRef]
  11. C. C. H. Petersen, A. Grinvald, and B. Sakmann, J. Neurosci. 23, 1298 (2003). [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited