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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 1 — Jan. 4, 2010

Enhanced resolution in two-photon imaging using a TM 01 laser beam at a dielectric interface

Harold Dehez, Michel Piché, and Yves De Koninck  »View Author Affiliations

Optics Letters, Vol. 34, Issue 23, pp. 3601-3603 (2009)

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We have experimentally demonstrated that the resolution of a commercial two-photon microscope is improved using a TM 01 laser beam. With a water immersion objective having a 1.2 NA, the measured point- spread function has an area of 0.15 λ 2 . We used a plane interface between dielectrics instead of an annular aperture to increase the relative contribution of the longitudinal field of the TM 01 laser beam. The results are in agreement with the vectorial diffraction theory established by Richards and Wolf [ Proc. R. Soc. Lond. A 253, 358 (1959) ]. The TM 01 laser beam was produced with a quadrant of half-wave plates. We have also used the same mode converter to generate a TE 01 laser beam with a zero at the beam center.

© 2009 Optical Society of America

OCIS Codes
(140.3300) Lasers and laser optics : Laser beam shaping
(180.5810) Microscopy : Scanning microscopy
(350.5730) Other areas of optics : Resolution
(140.3295) Lasers and laser optics : Laser beam characterization

ToC Category:
Lasers and Laser Optics

Original Manuscript: August 14, 2009
Revised Manuscript: October 19, 2009
Manuscript Accepted: October 19, 2009
Published: November 17, 2009

Virtual Issues
Vol. 5, Iss. 1 Virtual Journal for Biomedical Optics

Harold Dehez, Michel Piché, and Yves De Koninck, "Enhanced resolution in two-photon imaging using a TM01 laser beam at a dielectric interface," Opt. Lett. 34, 3601-3603 (2009)

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