OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 1 — Jan. 4, 2010

Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells

YongKeun Park, Toyohiko Yamauchi, Wonshik Choi, Ramachandra Dasari, and Michael S. Feld  »View Author Affiliations

Optics Letters, Vol. 34, Issue 23, pp. 3668-3670 (2009)

View Full Text Article

Enhanced HTML    Acrobat PDF (321 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We report a practical method for label-free quantification of specific molecules using spectroscopic imaging of sample-induced phase shifts. Diffraction phase microscopy equipped with various wavelengths of light source is used to record wavelength-dependent phase images. We first perform dispersion measurements on pure solutions of single molecular species present in the cells, such as albumin and hemoglobin (Hb). With this prior calibration of molecular specific dispersion, we demonstrate the extraction of Hb concentration from individual human red blood cells. The end point of this study is noninvasive monitoring of physiological states of intact living cells.

© 2009 Optical Society of America

OCIS Codes
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(180.0180) Microscopy : Microscopy

ToC Category:

Original Manuscript: August 25, 2009
Manuscript Accepted: October 15, 2009
Published: November 20, 2009

Virtual Issues
Vol. 5, Iss. 1 Virtual Journal for Biomedical Optics

YongKeun Park, Toyohiko Yamauchi, Wonshik Choi, Ramachandra Dasari, and Michael S. Feld, "Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells," Opt. Lett. 34, 3668-3670 (2009)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. R. Barer, Nature 172, 1097 (1953). [CrossRef] [PubMed]
  2. E. Cuche, F. Bevilacqua, and C. Depeursinge, Opt. Lett. 24, 291 (1999). [CrossRef]
  3. A. Barty, K. Nugent, D. Paganin, and A. Roberts, Opt. Lett. 23, 817 (1998). [CrossRef]
  4. C. G. Rylander, D. P. Davé, T. Akkin, T. E. Milner, K. R. Diller, and A. J. Welch, Opt. Lett. 29, 1509 (2004). [CrossRef] [PubMed]
  5. G. Popescu, T. Ikeda, R. R. Dasari, and M. S. Feld, Opt. Lett. 31, 775 (2006). [CrossRef] [PubMed]
  6. Y.-K. Park, M. Diez-Silva, G. Popescu, G. Lykotrafitis, W. Choi, M. S. Feld, and S. Suresh, Proc. Natl. Acad. Sci. USA 105, 13730 (2008). [CrossRef] [PubMed]
  7. B. Rappaz, F. Charriere, C. Depeursinge, P. J. Magistretti, and P. Marquet, Opt. Lett. 33, 744 (2008). [CrossRef] [PubMed]
  8. Y. Park, W. Choi, Z. Yaqoob, R. Dasari, K. Badizadegan, and M. Feld, Opt. Express 17, 12285 (2009). [CrossRef] [PubMed]
  9. B. Kemper and G. von Bally, Appl. Opt. 47, 52 (2008). [CrossRef]
  10. B. Rappaz, M. Marquet, E. Cuche, Y. Emery, C. Depeursinge, and P. J. Magistretti, Opt. Express 13, 9361 (2005). [CrossRef] [PubMed]
  11. G. Popescu, Y. Park, W. Choi, R. Dasari, M. Feld, and K. Badizadegan, Blood Cells Mol. Dis. 41, 10 (2008). [CrossRef] [PubMed]
  12. N. Shaked, M. Reinhart, and A. Wax, Opt. Lett. 34, 767 (2009). [CrossRef] [PubMed]
  13. G. Popescu, Y. Park, N. Lue, C. Best-Popescu, L. Deflores, R. Dasari, M. Feld, and K. BadizadeganAm. J. Physiol.: Cell Physiol. 295, C538 (2008). [CrossRef]
  14. C. Yang, A. Wax, I. Georgakoudi, E. Hanlon, K. Badizadegan, R. Dasari, and M. Feld, Opt. Lett. 25, 1526 (2000). [CrossRef]
  15. Y. K. Park, G. Popescu, K. Badizadegan, R. R. Dasari, and M. S. Feld, Opt. Express 14, 8263 (2006). [CrossRef] [PubMed]
  16. P. Schiebener, J. Straub, J. Sengers, and J. Gallagher, J. Phys. Chem. Ref. Data 19, 677 (1990). [CrossRef]
  17. M. Andersen, L. Painter, and S. Nir, Biopolymers 13, 1261 (1974). [CrossRef]
  18. M. Friebel and M. Meinke, Appl. Opt. 45, 2838 (2006). [CrossRef] [PubMed]
  19. M. Lichtman, Williams Hematology (McGraw-Hill Professional, 2005).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited